Surface plasmon resonance/mass spectrometry interface
Surface plasmon resonance/mass spectrometry interface
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DOI：
10.1021/ac049033d
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发表时间：
2005-02-15
影响因子：
7.4
通讯作者：
Koenig, S
中科院分区：
文献类型：
--
作者：
Grote, J;Dankbar, N;Koenig, S
报道了一种结合表面等离子体共振（SPR）生物分子相互作用分析和基质辅助激光解吸/电离质谱（MALDI-MS）的方法。这两种技术是高度互补的，但需要单独优化，以提高其各自的特异性和灵敏度。对于动力学分析最佳的传感器表面不太适合MALDI-MS，反之亦然。此外，分析物从SPR到MS的转移是至关重要的，并且通常伴随着样品损失。为了解决这两个问题，构建了一个双功能SPR流体池，其中优化的表面可以用于结合研究和MS同时考虑到每种技术的特殊需要。该设置确保SPR和MS的加载实验在相同条件下进行。一个可移动的针携带亲和表面结合的分析物到质谱仪，使处理最小化，避免分析物洗脱。官能化的转移针也可以独立于SPR用于微亲和捕获-MS。
A strategy for combining surface plasmon resonance (SPR) biomolecular interaction analysis, and matrixassisted laser desorption/ionization mass spectrometry (MALDI-MS) is reported. Both techniques are highly complementary but need separate optimization to improve their individual specificity and sensitivity. Sensor surfaces that are optimal for kinetic analysis are not well suited for MALDI-MS and vice versa. In addition, the transfer of analyte from SPR to MS is crucial and often accompanied by sample loss. To address both of these points, a bifunctional SPR fluid cell was constructed where optimized surfaces can be used for binding studies and MS simultaneously with regard to the special need of each technique. The setup guarantees that the SPR and the loading experiment for MS are performed at identical conditions. A removable pin carries the affinity-surface-bound analyte to the mass spectrometer so that handling is minimized, avoiding analyte elution. Functionalized transfer pins can also be used independently of SPR for microaffinity capture-MS.