Mass Spectrometry-Based Metabolomics Reveals Paraben Exposure Related to Purine Metabolism and Other Pathways.
Mass Spectrometry-Based Metabolomics Reveals Paraben Exposure Related to Purine Metabolism and Other Pathways.
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DOI：
10.1021/acs.est.9b07634
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发表时间：
2020-02
影响因子：
11.4
通讯作者：
Hongzhi Zhao;Yuanyuan Zheng;Lin Zhu;Li Xiang;Yanqiu Zhou;Jiufeng Li;Jing Fang;Shunqing Xu
中科院分区：
文献类型：
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作者：
Hongzhi Zhao;Yuanyuan Zheng;Lin Zhu;Li Xiang;Yanqiu Zhou;Jiufeng Li;Jing Fang;Shunqing Xu
对羟基苯甲酸酯广泛用作制药和化妆品工业中的常用防腐剂。已发现暴露于对羟基苯甲酸酯与人体代谢改变和代谢疾病（如糖尿病）风险增加有关。然而，有限的信息是可利用的代谢途径有关的对羟基苯甲酸酯暴露。采用超高效液相色谱-三重四极杆质谱法（UHPLC-QqQ MS）对88例孕妇尿样中的3种尼泊金酯进行了检测。根据尿中对羟基苯甲酸酯浓度的三分位分布将样本分为不同的组。采用UHPLC结合Orbitrap高分辨率MS对88份尿样进行代谢分析。通过比较不同对羟基苯甲酸酯暴露组尿样的代谢谱，筛选差异代谢物。代谢产物包括嘌呤类、酰基肉毒碱类等，表明对羟基苯甲酸酯干扰了嘌呤代谢、脂肪酸β-氧化等代谢途径。18种和3种代谢物分别与对羟基苯甲酸甲酯和对羟基苯甲酸丙酯的暴露水平相关（斯皮尔曼相关性分析，p < 0.05）。这是第一个基于MS的非靶向代谢组学研究与对羟基苯甲酸酯暴露的孕妇。研究结果揭示了接触对羟基苯甲酸酯的潜在健康风险，并可能有助于了解对羟基苯甲酸酯接触和一些代谢疾病之间的联系。
Parabens are widely used as common preservatives in pharmaceutical and cosmetic industries. Exposure to parabens has been found to be associated with metabolic alterations of human and the increased risk of metabolic disease, such as diabetes. However, limited information is available about metabolic pathways related to paraben exposure. In this study, three parabens were determined in the urine samples of 88 pregnant women by using ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QqQ MS). The samples were divided into different groups based on tertile distribution of urinary paraben concentrations. Metabolic profiling of the 88 urine samples was performed by using UHPLC coupled with Orbitrap high-resolution MS. Differential metabolites were screened by comparing the profiles of urine samples from different paraben exposure groups. The identified metabolites included purines, acylcarnitines, etc, revealing that metabolic pathways such as purine metabolism, fatty acid β-oxidation and other pathways were disturbed by parabens. Eighteen and three metabolites were correlated (Spearman correlation analysis, p < 0.05) with the exposure levels of methyparaben and propylparaben, respectively. This is the first MS-based non-targeted metabolomics study on pregnant women with paraben exposure. The findings reveal the potential health risk of exposure to parabens and might help understand the link between paraben exposure and some metabolic disease.