LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition
LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition
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DOI：
10.1016/s0040-4020(98)00094-5
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发表时间：
1998-04-01
期刊：
影响因子：
2.1
通讯作者：
Wengel, J
中科院分区：
文献类型：
--
作者：
Koshkin, AA;Singh, SK;Wengel, J
由2 '-O，4'-C-亚甲基双环苷单体组成的LNA（锁核酸）被有效地合成，并且其核酸识别潜力被评估为六种不同的核碱基，即腺嘌呤、胞嘧啶、鸟嘌呤、5-甲基胞嘧啶、胸腺嘧啶和尿嘧啶。当评估部分或完全修饰的LNA的混合序列时，获得了双链体对DNA和RNA的热稳定性的前所未有的增加（每次修饰+3至+8 ℃）。错配序列的研究表明，LNA遵守沃森-克里克碱基配对规则，与相应的未修饰的参考链相比，选择性普遍提高。(C)1998爱思唯尔科技有限公司版权所有。
LNA (Locked Nucleic Acids), consisting of 2'-O,4'-C-methylene bicyclonucleoside monomers, is efficiently synthesized and its nucleic acid recognition potential evaluated for six different nucleobases, namely adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil. Unprecedented increases (+3 to +8 degrees C per modification) in the thermal stability of duplexes towards both DNA and RNA were obtained when evaluating mixed sequences of partly or fully modified LNA. Studies of mis-matched sequences show that LNA obey the Watson-Crick base pairing rules with generally improved selectivities compared to the corresponding unmodified reference strands. (C) 1998 Elsevier Science Ltd. All rights reserved.