Nuclear DNA content of the whitefly Bemisia tabaci (Aleyrodidae: Hemiptera) estimated by flow cytometry
Nuclear DNA content of the whitefly Bemisia tabaci (Aleyrodidae: Hemiptera) estimated by flow cytometry
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DOI：
10.1079/ber2005361
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发表时间：
2005-07-01
影响因子：
1.9
通讯作者：
Galbraith, D. W.
中科院分区：
文献类型：
--
作者：
Brown, J. K.;Lambert, G. M.;Galbraith, D. W.
用流式细胞术测定了烟粉虱的细胞核DNA含量。用碘化丙啶对雄核和雌核进行染色，以鸡红细胞和拟南芥(十字花科)为外标测定其DNA含量。雄性和雌性烟粉虱的核DNA含量估计分别为1.04和2.06 pg。这些结果证实了以往基于染色体计数的报道，即烟粉虱雄株为单倍体，雌株为二倍体。DNA含量与基因组大小的换算(1pg DNA=980Mbp)表明，烟粉虱单倍体基因组大小为1020Mbp，约为果蝇基因组大小的5倍。这些结果为烟粉虱复合体的基因组学研究提供了重要的基线。
The nuclear DNA content of the whitefly Bemisia tabaci (Gennnadius) was estimated using flow cytometry. Male and female nuclei were stained with propidium iodide and their DNA content was estimated using chicken red blood cells and Arabidopsis thaliana L. (Brassicaceae) as external standards. The estimated nuclear DNA content of male and female B. tabaci was 1.04 and 2.06 pg, respectively. These results corroborated previous reports based on chromosome counting, which showed that B. tabaci males are haploid and females are diploid. Conversion between DNA content and genome size (1 pg DNA = 980 Mbp) indicate that the haploid genome size of B. tabaci is 1020 Mbp, which is approximately five times the size of the genome of the fruitfly Drosophila melanogaster Meigen. These results provide an important baseline that will facilitate genomics-based research for the B. tabaci complex.