Stepwise Assembly of a Relaxosome at the F Plasmid Origin of Transfer (*)
Stepwise Assembly of a Relaxosome at the F Plasmid Origin of Transfer (*)
复制标题
在 F 质粒转移起点处逐步组装松弛体 (*)
DOI：
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发表时间：
1995
影响因子：
4.8
通讯作者：
S. W. Matson
中科院分区：
文献类型：
--
作者：
M. Howard;W. Nelson;S. W. Matson
A central step in the transfer of genetic information during bacterial conjugation of the Escherichia coli F plasmid involves the formation of a protein-DNA complex, called the relaxosome, at the origin of transfer. During conjugation, the relaxosome introduces a site- and strand-specific nick from which the physical transfer of a single strand of DNA is initiated. At least two F-encoded proteins, TraIp (traI gene product) and TraYp (traY gene product), and one host-encoded protein, integration host factor, are involved in this process. In this report, we use DNase I protection and electron microscopic techniques to investigate the mechanism of relaxosome formation. Our results show that TraYp and integration host factor form a protein-DNA complex that facilitates the binding of TraIp to assemble a relaxosome capable of introducing a site- and strand-specific nick at the origin of transfer. This nick is identical to that observed during conjugation.
