Ptychographic transmission microscopy in three dimensions using a multi-slice approach
Ptychographic transmission microscopy in three dimensions using a multi-slice approach
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DOI：
10.1364/josaa.29.001606
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发表时间：
2012-08-01
影响因子：
1.9
通讯作者：
Rodenburg, J. M.
中科院分区：
文献类型：
--
作者：
Maiden, A. M.;Humphry, M. J.;Rodenburg, J. M.
一般来说，三维成像的方法，如共焦显微镜和计算机断层扫描依赖于两个要素：多个测量（在一个范围内的焦点位置或旋转）和一个弱散射的标本（以避免在共焦显微镜焦斑失真或满足投影近似断层扫描）。在这里，我们表明，一种替代形式的多测量成像，重叠关联，可以扩展到三维，并可以成功地恢复图像中存在的多重散射时，投影近似不适用。我们用可见光实验证明了我们的技术，它在成像厚样品，如生物组织中的应用;然而，结果也有重要的影响，x射线和电子成像。(C)2012美国光学学会
Generally, methods of three-dimensional imaging such as confocal microscopy and computed tomography rely on two essentials: multiple measurements (at a range of focus positions or rotations) and a weakly scattering specimen (to avoid distortion of the focal spot in the confocal microscope or to satisfy the projection approximation in tomography). Here we show that an alternative form of multi-measurement imaging, ptychography, can be extended to three dimensions and can successfully recover images in the presence of multiple scattering and when the projection approximation is not applicable. We demonstrate our technique experimentally using visible light, where it has applications in imaging thick samples such as biological tissues; however the results also have important implications for x ray and electron imaging. (C) 2012 Optical Society of America