Metabolic cross-feeding in imbalanced diets allows gut microbes to improve reproduction and alter host behaviour
Metabolic cross-feeding in imbalanced diets allows gut microbes to improve reproduction and alter host behaviour
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DOI：
10.1038/s41467-020-18049-9
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发表时间：
2020-08-25
影响因子：
16.6
通讯作者：
Ribeiro, Carlos
中科院分区：
文献类型：
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作者：
Henriques, Silvia F.;Dhakan, Darshan B.;Ribeiro, Carlos
肠道细菌对宿主的影响来自复杂的微生物-饮食-宿主相互作用。因此，绘制肠道微生物群落中的代谢相互作用是了解微生物组如何影响宿主的关键。在这里，我们使用一个跨学科的方法，包括同位素分辨代谢组学表明，在果蝇，醋杆菌pomorum（AP）和植物乳杆菌（LP）的互养关系的建立，以克服有害的主机饮食和确定AP的细菌改变主机的喂养决定。具体来说，我们表明，Ap使用Lp产生的乳酸盐来供应Lp所必需的氨基酸，使其能够在不平衡的饮食中生长。乳酸盐也是必要的，足以让Ap改变苍蝇的蛋白质食欲。我们的数据表明，肠道细菌群落利用代谢相互作用来适应有害的宿主饮食。这些相互作用还确保了微生物组用于改变生殖和宿主行为的代谢物的不断流动。
The impact of commensal bacteria on the host arises from complex microbial-diet-host interactions. Mapping metabolic interactions in gut microbial communities is therefore key to understand how the microbiome influences the host. Here we use an interdisciplinary approach including isotope-resolved metabolomics to show that in Drosophila melanogaster, Acetobacter pomorum (Ap) and Lactobacillus plantarum (Lp) a syntrophic relationship is established to overcome detrimental host diets and identify Ap as the bacterium altering the host's feeding decisions. Specifically, we show that Ap uses the lactate produced by Lp to supply amino acids that are essential to Lp, allowing it to grow in imbalanced diets. Lactate is also necessary and sufficient for Ap to alter the fly's protein appetite. Our data show that gut bacterial communities use metabolic interactions to become resilient to detrimental host diets. These interactions also ensure the constant flow of metabolites used by the microbiome to alter reproduction and host behaviour.