Activity assays for receptor-interacting protein kinase 1:a key regulator of necroptosis.
Activity assays for receptor-interacting protein kinase 1:a key regulator of necroptosis.
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DOI：
10.1007/978-1-62703-383-1_3
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发表时间：
2013-01-01
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影响因子：
--
通讯作者：
Degterev, Alexei
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文献类型：
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作者：
Maki, Jenny L;Degterev, Alexei
坏死性上睑下垂是一种受调控的非凋亡细胞死亡的新形式，表现为坏死的形态学特征。受体相互作用蛋白激酶1 （RIP1）的激酶活性是坏死性坏死信号传导的关键组成部分。开发评估RIP1激酶活性的检测方法对于进一步开发现有的和新的坏死性坏死抑制剂非常重要。在这里，我们描述了RIP1蛋白在哺乳动物和昆虫细胞中的表达和纯化，以及两种检测RIP1激酶活性和抑制作用的体外激酶实验。
Necroptosis is a novel form of regulated non-apoptotic cell death, which displays morphological features of necrosis. The kinase activity of receptor-interacting protein kinase 1 (RIP1) is a critical component in signaling for necroptosis. The development of assays to evaluate RIP1 kinase activity is important in the further development of existing and novel inhibitors of necroptosis. Here, we describe RIP1 protein expression and purification from mammalian and insect cells as well as two in vitro kinase assays to detect RIP1 kinase activity and inhibition.