P-31 NMR saturation transfer study of the creatine kinase reaction in human skeletal muscle at rest and during exercise
P-31 NMR saturation transfer study of the creatine kinase reaction in human skeletal muscle at rest and during exercise
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DOI：
10.1002/mrm.1910370518
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发表时间：
1997-05-01
影响因子：
3.3
通讯作者：
Sturbois, X
中科院分区：
文献类型：
--
作者：
Goudemant, JF;Francaux, M;Sturbois, X
通过 P-31 NMR 在锻炼人小腿肌肉时研究了肌酸激酶反应，在休息和运动期间进行了高能磷酸盐的定量分析和 ATP 合成 (V-for) 方向的肌酸激酶通量的饱和转移研究。正如预期的那样，运动引起 [PCr] 降低（从 28.5 +/- 0.9 到 21.9 +/- 1.5 mM，P < 0.01），同时 P-i 增加（从 4.5 +/- 0.2 到 8.9 +/- 1.8 mM，P = 0.06）。 pH(i) 和 [ATP] 保持不变。 V-for 从休息 (12.4 +/- 0.9 mM s(-1)) 到中等运动水平没有变化，并在最高运动水平 (8.4 +/- 1.4 mM s(-1)，P = 0.006) 时下降。这一观察结果与肌酸激酶速率方程的预测不同，显示通量随着运动强度的增加而增加，计算表明这种差异是由代谢物区室化和/或由平面阴离子稳定的死端复合物的反应动力学引起的。
The creatine kinase reaction has been studied by P-31 NMR in exercising human calf muscle, Quantitative analysis of high energy phosphates and saturation transfer study of the creatine kinase flux in the direction of ATP synthesis (V-for) were performed at rest and during exercise. As expected, exercise induced a [PCr] decrease (from 28.5 +/- 0.9 to 21.9 +/- 1.5 mM, P < 0.01) matched by a P-i increase (from 4.5 +/- 0.2 to 8.9 +/- 1.8 mM, P = 0.06). pH(i) and [ATP] remained unchanged. V-for did not change from rest (12.4 +/- 0.9 mM s(-1)) to moderate exercise and decreased at the highest exercise level (8.4 +/- 1.4 mM s(-1), P = 0.006). This observation differs from the prediction of the creatine kinase rate equation, showing an increase in the flux with exercise intensity, computations suggest that this discrepancy arises from metabolite compartmentalization and/or from the reaction kinetics of a dead end complex stabilized by planar anions.