Hypercytokinemia in COVID-19: Tear cytokine profile in hospitalized COVID-19 patients
Hypercytokinemia in COVID-19: Tear cytokine profile in hospitalized COVID-19 patients
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DOI：
10.1016/j.exer.2020.108253
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发表时间：
2020-11-01
影响因子：
3.4
通讯作者：
Maria Martinez-de-la-Casa, Jose
中科院分区：
文献类型：
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作者：
Burgos-Blasco, Barbara;Guemes-Villahoz, Noemi;Maria Martinez-de-la-Casa, Jose
本研究的目的是分析住院COVID-19患者泪液中细胞因子的浓度与健康对照组的比较。41名健康对照和62名COVID-19患者的泪液样本。评估27种细胞因子：白细胞介素(IL)-1b、IL- 1ra、IL-2、IL-4、IL-5、IL-6、IL-7、IL-8、IL-9、IL-10、IL-12、IL-13、IL-15、IL-17、eotaxin、成纤维细胞生长因子基础、粒细胞集落刺激因子（G-CSF）、粒细胞-单核细胞集落刺激因子（GM-CSF）、干扰素(IFN)- γ、干扰素- γ诱导蛋白、单核细胞化学引诱蛋白-1、巨噬细胞炎症蛋白(MIP)-1a、MIP-1b、血小板衍生生长因子（PDGF），在活化正常T细胞上表达和分泌；肿瘤坏死因子- α和血管内皮生长因子（VEGF）。在COVID-19患者泪液样本中，与对照组相比，IL-9、IL-15、G-CSF、GM-CSF、ifn - γ、PDGF和VEGF升高，eotaxin降低（p < 0.05）。泪液和血液中IL-6水平的相关性较差。IL-1RA和GM-CSF在重症患者和需要免疫系统治疗的患者中显著降低（p < 0.05）。泪液细胞因子水平证实了SARS-CoV-2的炎症性。
The aim of this study is to analyze the concentrations of cytokines in tear of hospitalized COVID-19 patients compared to healthy controls. Tear samples were obtained from 41 healthy controls and 62 COVID-19 patients. Twenty-seven cytokines were assessed: interleukin (IL)-1b, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, eotaxin, fibroblast growth factor basic, granulocyte colony-stimulating factor (G-CSF), granulocyte-monocyte colony-stimulating factor (GM-CSF), interferon (IFN)-gamma, interferon gamma-induced protein, monocyte chemo-attractant protein-1, macrophage inflammatory protein (MIP)-1a, MIP-1b, plateletderived growth factor (PDGF), regulated on activation normal T cell expressed and secreted, tumor necrosis factor-alpha and vascular endothelial growth factor (VEGF).In tear samples of COVID-19 patients, an increase in IL-9, IL-15, G-CSF, GM-CSF, IFN-gamma, PDGF and VEGF was observed, along with a decrease in eotaxin compared to the control group (p < 0.05). A poor correlation between IL-6 levels in tear and blood was found. IL-1RA and GM-CSF were significantly lower in severe patients and those who needed treatment targeting the immune system (p < 0.05). Tear cytokine levels corroborate the inflammatory nature of SARS-CoV-2.