Evidence for the prion hypothesis: induction of the yeast [PSI+] factor by in vitro- converted Sup35 protein.
Evidence for the prion hypothesis: induction of the yeast [PSI+] factor by in vitro- converted Sup35 protein.
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朊病毒假说的证据：体外转化的 Sup35 蛋白诱导酵母 [PSI] 因子。
DOI：
10.1126/science.289.5479.595
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发表时间：
2000
期刊：
影响因子：
56.9
通讯作者：
J. Weissman
中科院分区：
文献类型：
--
作者：
H. Sparrer;Alex Santoso;F. Szoka;J. Weissman
从纯化的细菌产生的蛋白质开始，我们基于酵母 Sup35 蛋白质的改变（朊病毒）构象创建了一种 [PSI(+)] 诱导剂。在体外将 Sup35p 转化为其朊病毒构象后，我们使用脂质体转化方案将其引入活酵母的细胞质中。引入亚化学计量的转化 Sup35p 大大提高了已充分表征的表观遗传因子 [PSI+] 的出现率，该因子是细胞 Sup35p 自我繁殖聚集体的结果。因此，正如朊病毒假说所预测的那样，蛋白质可以通过引起自我传播的构象变化而充当传染原。
Starting with purified, bacterially produced protein, we have created a [PSI(+)]-inducing agent based on an altered (prion) conformation of the yeast Sup35 protein. After converting Sup35p to its prion conformation in vitro, we introduced it into the cytoplasm of living yeast using a liposome transformation protocol. Introduction of substoichiometric quantities of converted Sup35p greatly increased the rate of appearance of the well-characterized epigenetic factor [PSI+], which results from self-propagating aggregates of cellular Sup35p. Thus, as predicted by the prion hypothesis, proteins can act as infectious agents by causing self-propagating conformational changes.
影响酿酒酵母中[PSI]朊病毒从头出现的遗传和环境因素。
影响因子：
3.3
作者：
Derkatch,IL;Bradley,ME;Zhou,P;Chernoff,YO;Liebman,SW
通讯作者：
Liebman,SW