A high-throughput fluorescence screen to monitor the specific binding of antagonists to RNA targets
A high-throughput fluorescence screen to monitor the specific binding of antagonists to RNA targets
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DOI：
10.1006/abio.1998.2740
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发表时间：
1998-08-01
影响因子：
2.9
通讯作者：
Rando, RR
中科院分区：
文献类型：
--
作者：
Hamasaki, K;Rando, RR
由于RNA分子可以形成复杂的三维结构，因此应该可以设计针对这些结构的特异性高亲和力拮抗剂。为了开始探索这种可能性的有效性，需要高通量筛选方法来测定RNA拮抗剂。本文描述了在96孔板格式中的荧光猝灭技术，其能够筛选化学多样性文库。含芘的氨基糖苷类似物用于准确监测拮抗剂与原核16S rRNA A位点解码区构建体的结合。该rRNA区域包含氨基糖苷类抗生素的天然靶标。本文报道的荧光技术一般适用于监测结构新颖的拮抗剂与任何选定的RNA靶点的结合。(C)北京：科学出版社.
Since RNA molecules can form intricate three-dimensional structures, it should be possible to design specific, high-affinity antagonists directed against these structures. To begin to explore the validity of this possibility, high-throughput screening methods are required to assay for RNA antagonists. A fluorescence quenching technique is described here in a 96-well plate format which is capable of screening chemical diversity libraries, A pyrene-containing aminoglycoside analog is used to accurately monitor antagonist binding to a prokaryotic 16S rRNA A-site decoding region construct. This rRNA region comprises the natural target for aminoglycoside antibiotics. The fluorescence technique reported here should be generally adaptable to monitor the binding of structurally novel antagonists to any selected RNA target. (C) 1998 Academic Press.