Highly Fluorescent Ribonuclease-A-Encapsulated Lead Sulfide Quantum Dots for Ultrasensitive Fluorescence in Vivo Imaging in the Second Near-Infrared Window.
Highly Fluorescent Ribonuclease-A-Encapsulated Lead Sulfide Quantum Dots for Ultrasensitive Fluorescence in Vivo Imaging in the Second Near-Infrared Window.
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高荧光核糖核酸酶 A 封装的硫化铅量子点，用于第二近红外窗口体内超灵敏荧光成像
DOI：
10.1021/acs.chemmater.6b00208
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发表时间：
2016-05-10
期刊：
影响因子：
--
通讯作者：
Zhou D
中科院分区：
文献类型：
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作者：
Kong Y;Chen J;Fang H;Heath G;Wo Y;Wang W;Li Y;Guo Y;Evans SD;Chen S;Zhou D
核糖核酸酶-A（RNase-A）封装的PbS量子点（RNase-A@PbS Qdots）在第二近红外生物窗口（NIR-II，ca. 1000-1400 nm）在微波加热下快速合成。量子点的光致发光（PL）光谱可以通过简单地控制合成温度在整个NIR-II范围内调谐。通过透射电子显微镜（TEM）、原子力显微镜（AFM）和动态光散射（DLS）检测量子点的尺寸和形态。量子产率（Φf）测量证实所制备的量子点是用于体内成像的最亮的水溶性NIR-II发射体之一。它们的高Φf（17.3%）和1300 nm处的峰值发射确保了对肌肉组织的深度光学穿透（高达1.5 cm），并在每只小鼠15.2 pmol（101 μg）的极低阈值剂量下具有出色的成像对比度。重要的是，这种蛋白质包被的Qdot在体外对模型神经元、正常细胞和癌细胞没有显示出毒性迹象。此外，动物的代谢导致静脉注射的量子点在几天内通过网状内皮系统（RES）从体内彻底消除，这最大限度地减少了铅含量可能释放的潜在体内长期毒性。凭借高亮度、强大的光稳定性和优异的生物相容性等吸引人的特性，这种新型NIR-II发射Qdot在准确的疾病筛查和诊断应用中极具前景。
Ribonuclease-A (RNase-A) encapsulated PbS quantum dots (RNase-A@PbS Qdots) which emit in the second near-infrared biological window (NIR-II, ca. 1000–1400 nm) are rapidly synthesized under microwave heating. Photoluminescence (PL) spectra of the Qdots can be tuned across the entire NIR-II range by simply controlling synthesis temperature. The size and morphology of the Qdots are examined by transmission electron microscopy (TEM), atomic force microscopy (AFM), and dynamic light scattering (DLS). Quantum yield (Φf) measurement confirms that the prepared Qdots are one of the brightest water-soluble NIR-II emitters for in vivo imaging. Their high Φf (∼17.3%) and peak emission at ∼1300 nm ensure deep optical penetration to muscle tissues (up to 1.5 cm) and excellent imaging contrast at an extremely low threshold dose of ∼5.2 pmol (∼1 μg) per mouse. Importantly, this protein coated Qdot displays no signs of toxicity toward model neuron, normal, and cancer cells in vitro. In addition, the animal’s metabolism results in thorough elimination of intravenously injected Qdots from the body within several days via the reticuloendothelial system (RES), which minimizes potential long-term toxicity in vivo from possible release of lead content. With a combination of attractive properties of high brightness, robust photostability, and excellent biocompatibility, this new NIR-II emitting Qdot is highly promising in accurate disease screening and diagnostic applications.