Early inner retinal dysfunction in streptozotocin-induced diabetic rats
Early inner retinal dysfunction in streptozotocin-induced diabetic rats
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DOI：
10.1167/iovs.08-1679
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发表时间：
2008-08-01
影响因子：
4.4
通讯作者：
Bui, Bang V.
中科院分区：
文献类型：
--
作者：
Kohzaki, Kenichi;Vingrys, Algis J.;Bui, Bang V.
目的.已知糖尿病改变视网膜功能，如用视网膜电图（ERG）测量的，其显示视网膜内振荡电位（OPs）异常的倾向。然而，糖尿病对其他神经节细胞相关反应的影响尚不清楚。本研究系统评价了链脲佐菌素（STZ）致糖尿病大鼠11周内视网膜电图（ERG）的变化。将30只6周龄的Sprague-Dawley大鼠随机分配至治疗组（50 mg/kg STZ，n = 16）和对照组（1 mL/kg柠檬酸盐缓冲液，n = 14）。2只对照动物和4只STZ动物因血糖标准或全身并发症而被排除。糖尿病动物每天皮下注射1 - 2单位长效胰岛素。在治疗后4、8和11周测量ERG。a波被用作外视网膜功能的指标，而b波、OPs和暗视阈值反应（STR）被用作内视网膜功能的指标。光感受器（a-波）和双极细胞（b-波）的反应没有显着减少STZ治疗。OP在8周时显著降低（-25% +/-7%，P < 0.05）。受影响最严重的成分是以神经节细胞为主的阳性STR，从第一个时间点开始显著下降（4周时为-51% +/-11%，P < 0.05），但阴性成分在11周期间未受影响。神经节细胞为主的pSTR在STZ处理的大鼠中显示出大的损失。
PURPOSE. Diabetes is known to alter retinal function, as measured with the electroretinogram (ERG), which shows a propensity toward inner retinal oscillatory potential (OPs) abnormalities. However, the effect that diabetes has on other ganglion cell-related responses is not known. This study was a systematic evaluation of streptozotocin (STZ) diabetes-related ERG changes in rats for the first 11 weeks after diabetogenesis.METHODS. Thirty Sprague-Dawley rats were randomly assigned to treated (50 mg/kg STZ (n = 16) and control groups (1 mL/kg citrate buffer, n = 14) at 6 weeks of age. Two control animals and four STZ animals were excluded because of blood glucose criteria or systemic complications. Diabetic animals were given daily SC injections of 1 to 2 units of long-acting insulin. ERGs were measured at 4, 8, and 11 weeks after treatment. The a-wave was used as an index of outer retinal function, whereas the b-wave, OPs, and the scotopic threshold response (STR) were used as indices of inner retinal function.RESULTS. Photoreceptoral (a-wave) and bipolar cell (b-wave) responses were not significantly reduced by STZ treatment. OPs were significantly reduced by 8 weeks (-25% +/- 7%, P < 0.05). The most severely affected component was the ganglion cell-dominated positive STR, which was significantly decreased from the first time point (-51% +/- 11% at 4 weeks, P < 0.05), but the negative component was unaffected over the 11-week period.CONCLUSIONS. The ganglion cell dominated pSTR showed large losses in STZ treated rats.