Longitudinal, 3D Imaging of Collagen Remodeling in Murine Hypertrophic Scars In Vivo Using Polarization-Sensitive Optical Frequency Domain Imaging
Longitudinal, 3D Imaging of Collagen Remodeling in Murine Hypertrophic Scars In Vivo Using Polarization-Sensitive Optical Frequency Domain Imaging
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DOI：
10.1038/jid.2015.399
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发表时间：
2016-01-01
影响因子：
6.5
通讯作者：
Bouma, Brett E.
中科院分区：
文献类型：
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作者：
Lo, William C. Y.;Villiger, Martin;Bouma, Brett E.
增生性瘢痕（HTS），经常看到创伤性损伤和手术后，仍然是一个主要的临床挑战，因为现有的治疗方法的成功有限。理解HTS病因的一个重要障碍是缺乏纵向和非侵入性监测瘢痕重塑的工具。我们提出了一种在体内，无标记的技术，使用偏振敏感的光学频域成像的三维，纵向评估小鼠HTS的胶原蛋白重塑。在本研究中，在切口伤口上用机械张力装置诱导HTS 4-10天，并在装置取出后长达1个月进行成像;在损伤后6个月还对切除HTS模型进行成像，以研究更深和更成熟的疤痕。我们发现，本地延迟和偏振度提供了一个强大的签名HTS。与具有异质性局部延迟和低偏振度的正常皮肤相比，HTS的特征在于最初的低局部延迟，随着胶原纤维重塑而增加，以及持续的高偏振度。这项研究表明，偏振敏感的光频域成像提供了一个强大的工具，以获得显着的生物学见解HTS重塑，使纵向评估胶原蛋白在体内，这是至关重要的阐明HTS病因和开发更有效的HTS疗法。
Hypertrophic scars (HTS), frequently seen after traumatic injuries and surgery, remain a major clinical challenge because of the limited success of existing therapies. A significant obstacle to understanding HTS etiology is the lack of tools to monitor scar remodeling longitudinally and noninvasively. We present an in vivo, label-free technique using polarization-sensitive optical frequency domain imaging for the 3D, longitudinal assessment of collagen remodeling in murine HTS. In this study, HTS was induced with a mechanical tension device for 4-10 days on incisional wounds and imaged up to 1 month after device removal; an excisional HTS model was also imaged at 6 months after injury to investigate deeper and more mature scars. We showed that local retardation and degree of polarization provide a robust signature for HTS. Compared with normal skin with heterogeneous local retardation and low degree of polarization, HTS was characterized by an initially low local retardation, which increased as collagen fibers remodeled, and a persistently high degree of polarization. This study demonstrates that polarization-sensitive optical frequency domain imaging offers a powerful tool to gain significant biological insights into HTS remodeling by enabling longitudinal assessment of collagen in vivo, which is critical to elucidating HTS etiology and developing more effective HTS therapies.