Effects of prenatal exposure to antithyroid drugs on imprinting behavior in chicks
Effects of prenatal exposure to antithyroid drugs on imprinting behavior in chicks
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DOI：
10.1016/j.physbeh.2010.05.015
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发表时间：
2010-09-01
影响因子：
2.9
通讯作者：
Nishigori, Hideo
中科院分区：
文献类型：
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作者：
Kagami, Keisuke;Nishigori, Hidekazu;Nishigori, Hideo
甲状腺激素在脊椎动物大脑发育中起着重要作用。然而，对于胎儿甲状腺功能障碍对学习能力的直接影响，即不是通过母亲获得的，人们知之甚少。在本研究中，我们使用鸡胚胎作为胚胎模型，研究产前暴露于抗甲状腺药物对孵化雏鸡印记行为的影响。第14天分别以20mU/只或5mU/只丙基硫氧嘧啶(PTU)的剂量注射他巴唑(MMI)，对照组只给予赋形剂。在小鸡孵化后进行了印记测试。将1日龄雏鸡暴露在旋转训练对象中150min。第二天，训练过的雏鸟接触到训练对象和一个新奇的对象。印记偏好被表示为偏好分数(PS)，该偏好分数(PS)被计算为跟随训练对象相对于跟随训练对象和新对象的比率。MMI组PS为0.68+/-0.06(范围0.38-0.88)，显著低于对照组(0.86+/-0.04，P<0.05)。
Thyroid hormones play important roles in vertebrate brain development. However, there is little understanding of the direct effects of fetal thyroid dysfunction, i.e., not acquired through the mother, on learning ability. In the present study, we use a chick embryo as a fetal model to investigate the effects of prenatal exposure to antithyroid drugs on imprinting behavior in hatched chicks. Methimazole (MMI) at 20 mu mol/egg or 5 mu mol/egg of propylthiouracil (PTU) was administered to eggs on day 14 while the control was given only a vehicle. An imprinting test was conducted after the chicks hatched. Day-old chicks were exposed to a rotating training object for 150 min. The next day, the trained chicks were exposed to the training object and a novel object. The imprinting preference was represented as a preference score (PS) calculated as the rate of following the training object to following the training and novel objects. In the MMI-treated chicks, the PS was 0.68 +/- 0.06 (range, 0.38-0.88), which was significantly lower than that in the control chicks (0.86 +/- 0.04, p