Quantitation of staphylococcal protein A: Determination of equilibrium constant and number of protein A residues on bacteria.
Quantitation of staphylococcal protein A: Determination of equilibrium constant and number of protein A residues on bacteria.
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葡萄球菌蛋白 A 的定量：平衡常数和细菌上蛋白 A 残留数的测定。
DOI：
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发表时间：
1970
影响因子：
4.4
通讯作者：
Ralph C. Williams
中科院分区：
文献类型：
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作者：
G. Kronvall;P. Quie;Ralph C. Williams
为定量细胞壁相关的葡萄球菌蛋白A的方法开发使用125碘标记的骨髓瘤球蛋白。该方法的最大灵敏度可以根据特定的实验问题而变化。使用相同的试剂，估计金黄色葡萄球菌科万I上的蛋白A残基数量为80，000/微生物。与血型抗原A相比，蛋白A残基在该葡萄球菌菌株上的密度是其四倍。两种骨髓瘤球蛋白与葡萄球菌蛋白A反应的平衡常数分别为4.0 × 107和4.5 × 107升/摩尔。
For quantitation of cell wall associated staphylococcal protein A a method was developed using 125I-labeled myeloma globulins. The maximal sensitivity of the method could be varied according to the particular experimental questions. Using the same reagent the number of protein A residues on Staphylococcus aureus, Cowan I, was estimated to 80,000/organism. In comparison with the blood group antigen A the protein A residue is four times as densely located on this staphylococcal strain. The equilibrium constants for the reactions between two myeloma globulins and staphylococcal protein A were found to be 4.0 × 107 and 4.5 × 107 liters/mol, respectively.