Intracellular chloride concentration of the mouse vomeronasal neuron.
Intracellular chloride concentration of the mouse vomeronasal neuron.
复制标题
DOI：
10.1186/s12868-015-0230-y
复制
发表时间：
2015-12-15
期刊：
影响因子：
2.4
通讯作者：
Yu CR
中科院分区：
文献类型：
--
作者：
Kim S;Ma L;Unruh J;McKinney S;Yu CR
犁鼻器（vomeronasal organ，VNO）是一种专门检测环境中信息素和异源性线索的器官。最近的研究表明，在VNO信号转导的多个离子通道，包括钙激活的氯离子通道（CACCs）的参与。CACC的开放似乎通过Cl−离子的流出导致VNO神经元的激活。然而，细胞内的Cl−浓度仍然无法确定。我们使用氯离子淬灭染料MQAE测量活VNO切片中VNO神经元的细胞内Cl−浓度。VNO神经元中的静息Cl−浓度为84.73 mM。尿液激活VNO神经元会导致Cl−浓度下降，这与Cl−流出以使细胞去极化的概念一致。对瞬时受体电位典型通道2（TRPC 2）缺失小鼠的VNO神经元进行了类似的观察，其静息Cl−浓度为81 mM。VNO神经元在高细胞内Cl−浓度下休息，当氯离子通道打开时，这可能导致细胞去极化。这些结果也提供了额外的支持TRPC 2-独立的VNO激活途径。
The vomeronasal organ (VNO) is specialized in detecting pheromone and heterospecific cues in the environment. Recent studies demonstrate the involvement of multiple ion channels in VNO signal transduction, including the calcium-activated chloride channels (CACCs). Opening of CACCs appears to result in activation of VNO neuron through outflow of Cl− ions. However, the intracellular Cl− concentration remains undetermined. We used the chloride ion quenching dye, MQAE, to measure the intracellular Cl− concentration of VNO neuron in live VNO slices. The resting Cl− concentration in the VNO neurons is measured at 84.73 mM. Urine activation of the VNO neurons causes a drop in Cl− concentration, consistent with the notion of an efflux of Cl− to depolarize the cells. Similar observation is made for VNO neurons from mice with deletion of the transient receptor potential canonical channel 2 (TRPC2), which have a resting Cl− concentrations at 81 mM. The VNO neurons rest at high intracellular Cl− concentration, which can lead to depolarization of the cell when chloride channels open. These results also provide additional support of TRPC2-independent pathway of VNO activation.