Wavefront optimized nonlinear microscopy of ex vivo human retinas
Wavefront optimized nonlinear microscopy of ex vivo human retinas
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DOI：
10.1117/1.3369001
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发表时间：
2010-03-01
影响因子：
3.5
通讯作者：
Artal, Pablo
中科院分区：
文献类型：
--
作者：
Gualda, Emilio J.;Bueno, Juan M.;Artal, Pablo
研制了一种采用哈特曼-夏克（HS）波前传感器控制超快激光束波前像差的多光子显微镜。该仪器使我们能够研究激光束像差对人视网膜组织双光子自身荧光成像的影响。我们证明了在波前控制的同时，通过调整激光系统腔体使光束像差最小化，从而改善了非线性显微成像。在不需要固定或染色程序的情况下，首次检测到几种人类视网膜解剖特征的非线性信号。除了提高图像质量之外，这种方法还降低了所需的激发功率水平，最大限度地减少了成像样品内的光毒性副作用。特别是，这对于研究健康和病变视网膜的生理和功能可能是重要的。2010年光学仪器工程师学会。(DOI: 10.1117/1.3369001)
A muhiphoton microscope incorporating a Hartmann-Shack (HS) wavefront sensor to control the ultrafast laser beam's wavefront aberrations has been developed. This instrument allowed us to investigate the impact of the laser beam aberrations on two-photon autofluorescence imaging of human retinal tissues. We demonstrated that nonlinear microscopy images are improved when laser beam aberrations are minimized by realigning the laser system cavity while wavefront controlling. Nonlinear signals from several human retinal anatomical features have been detected for the first time, without the need of fixation or staining procedures. Beyond the improved image quality, this approach reduces the required excitation power levels, minimizing the side effects of phototoxicity within the imaged sample. In particular, this may be important to study the physiology and function of the healthy and diseased retina. 2010 Society or Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3369001]