Release of Citric Acid into the Medium by Aluminum-Tolerant Carrot Cells
Release of Citric Acid into the Medium by Aluminum-Tolerant Carrot Cells
复制标题
耐铝胡萝卜细胞将柠檬酸释放到培养基中
DOI：
10.1093/oxfordjournals.pcp.a076782
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发表时间：
1984
影响因子：
4.9
通讯作者：
K. Ohira
中科院分区：
文献类型：
--
作者：
K. Ojima;H. Abé;K. Ohira
从培养的胡萝卜细胞系(SO-1)中筛选出的耐铝细胞系(TA-1)的一个生理特征是比亲本的SO-1细胞释放更多的柠檬酸。在培养基中加入一定浓度的氯化铝，使SO-1和TA-1能够正常生长而不受抑制。测定了发酵液中柠檬酸和可溶性铝的含量。在培养的前半段，大量柠檬酸从TA-1细胞释放到培养液中。在最大生长时，TA-1细胞培养液中柠檬酸的含量是SO-1细胞培养液中柠檬酸含量的两倍。通过添加AlCl3作为铝源，在培养过程中形成的铝化合物(S)沉淀随着培养过程的进行而变得可溶，这取决于培养基中柠檬酸的含量。
One physiological characteristic of an Al-tolerant cell line (TA-1) selected from a cultured carrot cell line (SO-1) was the release of more citric acid into the medium than the parental SO-1 line.Aluminum chloride was added to the media at a concentration, at which SO-1 as well as TA-1 could grow normally without inhibition. The amounts of citric acid and the soluble Al present in the medium were determined during the growth period. Much citric acid was released from TA-1 cells into the medium in the first half of the culture period. At the time of maximum growth, the amount of citric acid in the medium of TA-1 cells was twice as much as in the medium of SO-1 cells. The precipitates of Al compound(s), which were formed in the medium by the addition of AlCl3as the Al source, became soluble as culture proceeded, depending on the amount of citric acid present in the medium.