High-Fat Diet Delays and Fasting Advances the Circadian Expression of Adiponectin Signaling Components in Mouse Liver
High-Fat Diet Delays and Fasting Advances the Circadian Expression of Adiponectin Signaling Components in Mouse Liver
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DOI：
10.1210/en.2008-0944
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发表时间：
2009-01-01
期刊：
影响因子：
4.8
通讯作者：
Froy, Oren
中科院分区：
文献类型：
--
作者：
Barnea, Maayan;Madar, Zecharia;Froy, Oren
生物钟通过调节参与新陈代谢的酶的昼夜表达和/或活性来控制能量动态平衡。昼夜节律的紊乱可能会导致肥胖和代谢紊乱。我们测试了生物钟是否控制肝脏中的脂联素信号通路，以及禁食和/或高脂饮食是否影响这一控制。给小鼠喂食低脂或HF饲料，最后一天禁食。在RNA、蛋白质或酶活性水平上检测肝脏中Clock基因和脂联素代谢途径组分的昼夜节律性表达。此外，还检测了血糖、脂联素和胰岛素水平。在低脂饮食条件下，脂联素信号通路组分表现出昼夜节律性。然而，禁食和HF饮食改变了这种昼夜节律的表达；禁食导致了阶段提前，而HF饮食导致了阶段延迟。此外，一磷酸腺苷活化蛋白激酶水平在禁食期间较高，在HF饮食期间较低。HF饮食引起的时钟基因和脂联素信号通路各组成部分的时相和昼夜节律的改变可能会导致肥胖，并可能解释其他时钟控制的输出系统的中断，如血压和睡眠/醒来周期，通常与代谢紊乱有关。(内分泌学150：161-168,2009)
The circadian clock controls energy homeostasis by regulating circadian expression and/or activity of enzymes involved in metabolism. Disruption of circadian rhythms may lead to obesity and metabolic disorders. We tested whether the biological clock controls adiponectin signaling pathway in the liver and whether fasting and/or high-fat (HF) diet affects this control. Mice were fed low-fat or HF diet and fasted on the last day. The circadian expression of clock genes and components of adiponectin metabolic pathway in the liver was tested at the RNA, protein, or enzyme activity level. In addition, serum levels of glucose, adiponectin, and insulin were measured. Under low-fat diet, adiponectin signaling pathway components exhibited circadian rhythmicity. However, fasting and HF diet altered this circadian expression; fasting resulted in a phase advance, and HF diet caused a phase delay. In addition, adenosine monophosphate-activated protein kinase levels were high during fasting and low during HF diet. Changes in the phase and daily rhythm of clock genes and components of adiponectin signaling pathway as a result of HF diet may lead to obesity and may explain the disruption of other clock-controlled output systems, such as blood pressure and sleep/wake cycle, usually associated with metabolic disorders. (Endocrinology 150: 161-168, 2009)