Hematoma Changes During Clot Resolution After Experimental Intracerebral Hemorrhage.
Hematoma Changes During Clot Resolution After Experimental Intracerebral Hemorrhage.
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DOI：
10.1161/strokeaha.116.013146
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发表时间：
2016-06
期刊：
影响因子：
8.3
通讯作者：
Xi G
中科院分区：
文献类型：
--
作者：
Cao S;Zheng M;Hua Y;Chen G;Keep RF;Xi G
血肿清除发生在脑出血（ICH）后的几天，尚未得到很好的研究。在当前的研究中，我们检测了仔猪脑出血模型中血肿的变化。对去铁胺对血肿的影响也进行了研究。采用自体血注入仔猪右额叶诱导脑出血模型。首先，确定血肿变化的自然时间过程，最长可达7天。其次，观察去铁胺对血肿变化的影响。采用酶联免疫吸附法检测血肿血红蛋白和膜攻击复合物水平。免疫组织化学和Western blotting检测血块中的CD47（红细胞吞噬调节因子）、CD163（血红蛋白清扫剂受体）和血红素加氧酶-1（血红素降解酶）。脑出血后，血块内红细胞直径随时间减少。伴有膜攻击复合物积聚和血红蛋白水平降低。血肿发生红细胞吞噬，这与凝块CD47水平降低有关。活化的巨噬细胞/小胶质细胞CD163和血红素加氧酶-1阳性，随着时间的推移在凝块中积累。去铁胺治疗通过减少成员攻击复合物的形成和抑制凝块中CD47的损失来减弱血肿消退的过程。这些结果表明，膜攻击复合物和红细胞吞噬作用有助于脑出血后血肿清除，这可以通过去铁胺治疗而改变。
Hematoma clearance occurs in the days after intracerebral hemorrhage (ICH) and has not been well studied. In the current study, we examined changes in the hematoma in a piglet ICH model. The effect of deferoxamine on hematoma was also examined. The ICH model was induced by an injection of autologous blood into the right frontal lobe of piglets. First, a natural time course of hematoma changes up to 7 days was determined. Second, the effect of deferoxamine on hematoma changes was examined. Hemoglobin and membrane attack complex levels in the hematoma were examined by enzyme-linked immunosorbent assay. Immunohistochemistry and Western blotting was used to examine CD47 (a regulator of erythrophagocytosis), CD163 (a hemoglobin scavenger receptor) and heme oxygenase-1 (a heme degradation enzyme) in the clot. After ICH, there was a reduction in red blood cell diameter within the clot with time. This was accompanied by membrane attack complex accumulation and decreased hemoglobin levels. Erythrophagocytosis occurred in the hematoma and this was associated with reduced clot CD47 levels. Activated macrophages/microglia were CD163 and hemeoxygenase-1 positive and these accumulated in the clot with time. Deferoxamine treatment attenuated the process of hematoma resolution by reducing member attack complex formation and inhibiting CD47 loss in the clot. These results indicate that membrane attack complex and erythrophagocytosis contribute to hematoma clearance after ICH, which can be altered by deferoxamine treatment.