Improvement of an experimental colitis in rats by lactic acid bacteria producing superoxide dismutase
Improvement of an experimental colitis in rats by lactic acid bacteria producing superoxide dismutase
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DOI：
10.1097/01.mib.0000235101.09231.9e
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发表时间：
2006-11-01
影响因子：
4.9
通讯作者：
Fioramonti, Jean
中科院分区：
文献类型：
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作者：
Han, Wei;Mercenier, Annick;Fioramonti, Jean
超氧化物歧化酶（SOD）在炎症性疾病中的应用受到其短循环半衰期的阻碍。为了确定向结肠中供应SOD的细菌是否可以改善实验性结肠炎，比较了用产生不同量的SOD的活重组乳酸菌口服治疗的效果和结肠输注SOD的效果。在Wistar大鼠的近端结肠中安装导管，通过该导管施用TNBS以诱导结肠炎。动物接受连续结肠内输注牛SOD（40 U/大鼠每天）4天后TNBS或口服活重组乳酸乳球菌或植物乳杆菌菌株（109菌落形成单位（CFU）/天），生产或不生产SOD，4天前后TNBS。细菌提取液中SOD活性分别为0、26、74和624单位/10（9）CFU。[医]植物乳酸菌L. lactis SOD+和L. plantarum SOD+。TNBS后4天，对肉眼和显微镜下损伤、髓过氧化物酶（MPO）活性和硝基酪氨酸免疫染色进行评价。TNBS诱导宏观和微观损伤，MPO活性增加，和硝基酪氨酸的强烈免疫染色。牛SOD可减轻肝组织的大体损伤和MPO活性。L.乳酸菌L. lactis SOD+和L. plantarum SOD+，而L.植物用4种细菌菌株处理后，硝基酪氨酸免疫染色减弱。虽然不是所有的抗炎作用都可以直接归因于SOD，但我们的研究结果表明，SOD产生的乳酸菌在炎症性肠病治疗中开辟了一种新的方法。
The use of superoxide dismutases (SODs) in inflammatory diseases is hampered by their short circulatory half-life. To determine whether a bacterial supply of SOD into the colon might improve an experimental colitis, the effects of oral treatment with live recombinant lactic acid bacteria producing different amounts of SOD and those of colonic infusion of SOD were compared. Wistar rats were fitted with a catheter in the proximal colon through which TNBS was administered to induce colitis. Animals received a continuous intracolonic infusion of bovine SOD (40 U per rat per day) for 4 days after TNBS or were treated orally with live recombinant Lactococcus lactis or Lactobacillus plantarum strains (109 colony-forming units (CFU)/d), producing or not producing SOD, for 4 days before and after TNBS. SOD activity of bacterial extracts was 0, 26, 74, and 624 units/10(9) CFU for L. plantarum, L. lactis, L. lactis SOD+, and L. plantarum SOD+, respectively. Four days after TNBS, macroscopic and microscopic damage, myeloperoxidase (MPO) activity, and nitrotyrosine immunostaining were evaluated. TNBS induced macroscopic and microscopic damages, an increase in MPO activity, and intense immunostaining for nitrotyrosine. Macroscopic damage and MPO activity were reduced by bovine SOD. These parameters and microscopic damages also were reduced by L. lactis, L. lactis SOD+, and L. plantarum SOD+, but not by L. plantarum. Nitrotyrosine immunostaining was attenuated after treatment with the 4 bacterial strains. Although not all of the anti-inflammatory effects could be attributed directly to SOD, our results suggest that SOD-producing lactic acid bacteria open a novel approach in inflammatory bowel disease treatment.