Inhibitory Effects of Palmatine on P2X7 Receptor Expression in Trigeminal Ganglion and Facial Pain in Trigeminal Neuralgia Rats.
Inhibitory Effects of Palmatine on P2X7 Receptor Expression in Trigeminal Ganglion and Facial Pain in Trigeminal Neuralgia Rats.
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巴马汀对三叉神经节 P2X7 受体表达和三叉神经痛大鼠面部疼痛的抑制作用。
DOI：
10.3389/fncel.2021.672022
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发表时间：
2021
影响因子：
5.3
通讯作者：
Xiong W
中科院分区：
文献类型：
--
作者：
Yin C;Shen W;Zhang M;Wen L;Huang R;Sun M;Gao Y;Xiong W
三叉神经痛(TN)是指三叉神经分布区反复发作的严重阵发性疼痛，严重影响患者的生活质量。本研究采用慢性眶下神经压迫损伤(CCI-ION)的方法，建立了大鼠TN的动物模型。术后测定各组大鼠的机械痛阈值，用定量逆转录聚合酶链式反应、免疫荧光和免疫印迹法检测三叉神经节(TG)中P2X7受体的表达，用ELISA法检测大鼠血清中促炎症细胞因子IL-1β和肿瘤坏死因子-α的变化。结果表明，与假手术组相比，给予巴马汀可降低TN大鼠的机械痛阈值，显著降低TG内P2X7受体的表达，降低血清IL-1β和肿瘤坏死因子-α的浓度。此外，经巴马汀治疗后，TN大鼠TG中p38的磷酸化水平显著降低。同样，通过shRNA抑制P2X7的表达可以有效地对抗TN大鼠疼痛敏感性、IL-1β和肿瘤坏死因子-α的产生以及p38磷酸化的不利变化。提示巴马汀可能通过降低TN大鼠三叉神经节内P2X7受体的表达，抑制p38的磷酸化，减少IL-1β和肿瘤坏死因子-α的释放，从而减轻TN大鼠的机械性面部疼痛。
Trigeminal Neuralgia (TN) refers to recurrent severe paroxysmal pain in the distribution area of the trigeminal nerve, which seriously affects the quality of life of patients. This research applied the chronic constriction injury of the infraorbital nerve (CCI—ION) approach to induce an animal model of TN in rats. The mechanical pain threshold of each group of rats was determined postoperatively; the expression of P2X7 receptor in trigeminal ganglion (TG) was assessed by qRT-PCR, immunofluorescence and Western blot; and the changes of the proinflammatory cytokines IL-1β and TNF-α in serum of rats were detected by ELISA. The results showed that the administration of palmatine in the TN rats could reduce the mechanical pain threshold, significantly decrease the expression of P2X7 receptor in TG, and lower the serum concentrations of IL-1β and TNF-α, compared to the sham group. In addition, the phosphorylation level of p38 in TG of TN rats was significantly decreased after treatment with palmatine. Likewise, inhibition of P2X7 expression by shRNA treatment could effectively counteract the adversary changes of pain sensitivity, IL-1β and TNF-α production, and p38 phosphorylation in TN rats. Our data suggest that palmatine may alleviate mechanical facial pain in TN rats possibly by reducing the expression of P2X7 receptor in TG of TN rats, which may be attributable to inhibiting p38 phosphorylation and reducing the release of IL-1β and TNF-α.
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影响因子：
7.7
作者：
Kaczmarek-Hajek K;Zhang J;Kopp R;Grosche A;Rissiek B;Saul A;Bruzzone S;Engel T;Jooss T;Krautloher A;Schuster S;Magnus T;Stadelmann C;Sirko S;Koch-Nolte F;Eulenburg V;Nicke A
通讯作者：
Nicke A
影响因子：
9.3
作者：
He Y;Taylor N;Fourgeaud L;Bhattacharya A
通讯作者：
Bhattacharya A
巴马汀对 BDNF/TrkB 介导的三叉神经痛的影响。
影响因子：
4.6
作者：
Liu, Lijuan;He, Lingkun;Xiong, Wei
通讯作者：
Xiong, Wei
DOI：
10.1155/2016/7262054
发表时间：
2016
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Ishikawa S;Tamaki M;Ogawa Y;Kaneki K;Zhang M;Sunagawa M;Hisamitsu T
通讯作者：
Hisamitsu T
影响因子：
3.1
作者：
Huang, Bing;Yao, Ming;Qian, Xiang
通讯作者：
Qian, Xiang