Maintenance of neural stem cell regional identity in culture.
Maintenance of neural stem cell regional identity in culture.
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DOI：
10.1080/23262133.2016.1187321
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Lim, Daniel A
中科院分区：
文献类型：
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作者：
Delgado, Ryan N;Lu, Changqing;Lim, Daniel A
神经干细胞（NSCs）分布于成年小鼠大脑的整个脑室-室下区（V-SVZ）。位于V-SVZ空间上不同区域的NSCs产生不同类型的嗅球（OB）神经元，特定转录因子的区域表达与这些差异在NSC发育潜力上的相关性。在最近的一篇文章中，我们发现表达NKX2.1的胚胎前体可以产生位于成年小鼠腹侧V-SVZ的NKX2.1+ NSCs。在这里，我们描述了一个V-SVZ单层培养系统，它保留了来自V-SVZ背侧和腹侧的NSCs的区域基因表达和神经发生潜力。特别是，我们发现Nkx2.1谱系的V-SVZ NSCs通过序列传代维持Nkx2.1的表达，并能在体外产生新的神经元。因此，V-SVZ NSCs在培养中保留了其体内区域身份的关键方面，为理解这种发育模式在发育过程中如何建立和维持提供了新的实验机会。
Neural stem cells (NSCs) are distributed throughout the ventricular-subventricular zone (V-SVZ) in the adult mouse brain. NSCs located in spatially distinct regions of the V-SVZ generate different types of olfactory bulb (OB) neurons, and the regional expression of specific transcription factors correlates with these differences in NSC developmental potential. In a recent article, we show that Nkx2.1-expressing embryonic precursors give rise to NKX2.1+ NSCs located in the ventral V-SVZ of adult mice. Here we characterize a V-SVZ monolayer culture system that retains regional gene expression and neurogenic potential of NSCs from the dorsal and ventral V-SVZ. In particular, we find that Nkx2.1-lineage V-SVZ NSCs maintain Nkx2.1 expression through serial passage and can generate new neurons in vitro. Thus, V-SVZ NSCs retain key aspects of their in vivo regional identity in culture, providing new experimental opportunities for understanding how such developmental patterns are established and maintained during development.