High Expression of MDM2 and the p53 Protein is Predictive Biomarkers for Poor Prognosis of Oesophageal Squamous Cell Carcinoma.
High Expression of MDM2 and the p53 Protein is Predictive Biomarkers for Poor Prognosis of Oesophageal Squamous Cell Carcinoma.
复制标题
DOI：
10.2147/cmar.s280326
复制
发表时间：
2021
影响因子：
3.3
通讯作者：
Xie R
中科院分区：
文献类型：
--
作者：
Ye J;Zhang L;Li Z;Lin R;Song Y;Ni H;Gou X;Xie R
本研究检测MDM2和P53在食管鳞癌(OSCC)组织中的表达，探讨其与OSCC患者生存的关系，探讨MDM2和P53作为OSCC肿瘤标志物的可能性。采用免疫组织化学和荧光原位杂交技术，检测符合纳入和排除标准的157例口腔鳞癌组织中MDM2和P53蛋白的表达。评分后，采用皮尔逊卡方检验和COX回归分析。结果显示，口腔鳞癌组织中MDM2和P53高表达的比例分别为60.5%和51.0%。MDM2和P53在口腔鳞癌中的表达水平呈显著正相关(p<；0.001，r=0.414)。此外，口腔鳞癌的病理转移状态与mdm2蛋白表达显著相关(p=0.027)，P53蛋白的高表达与口腔鳞癌转移(p=0.005)、病理淋巴结状态(p=0.008)、临床分期(p=0.003)呈正相关。Kaplan-Meier生存分析显示MDM2和P53的高表达与口腔鳞癌的预后不良密切相关。此外，对口腔鳞癌患者TNM分期的亚组分析显示，无论是I-II期还是III-IV期，MDM2和P53的高表达与口腔鳞癌患者的OS和DFS均显著相关。单因素和COX多因素分析均显示P53和MDM2可作为影响口腔鳞癌患者预后的独立因素。最后，我们对mdm2的FISH检测结果显示mdm2的高表达与mdm2的扩增显著相关(p=0.015)。本研究表明MDM2和P53可作为食管鳞癌患者预后的独立预测因子。
In the present study, we detected the expression of MDM2 and p53 in oesophageal squamous cell carcinoma (OSCC) specimens, studied their relationship with the survival of OSCC patients, and explored the potential of MDM2 and p53 to serve as predictive OSCC tumour markers. Through immunohistochemistry and fluorescence in situ hybridization (FISH), we detected the expression of MDM2 and the p53 protein in 157 OSCC specimens that met the inclusion and exclusion criteria. After scoring the results, Pearson’s chi-square test and Cox regression were used for analysis. The results showed that the rates of high MDM2 and p53 expression in OSCC tissues were 60.5% and 51.0%, respectively. The expression levels of MDM2 and p53 in OSCC were significantly positively correlated (p<0.001, r=0.414). In addition, the pathological metastasis (M) status and MDM2 protein expression in OSCC were significantly correlated (p=0.027), and high expression of the p53 protein was positively correlated with OSCC transfer (p=0.005), pathological node status (p=0.008), and clinical stage (p=0.003). Kaplan-Meier survival analysis showed that the high expression of MDM2 and p53 was significantly related to the poor prognosis of OSCC. Moreover, subgroup analysis of the TNM staging of OSCC patients showed that the high expression of MDM2 and p53 was significantly correlated with poor OS and DFS of OSCC patients in either stage I–II or III–IV patients. Both univariate and Cox multivariate analyses showed that p53 and MDM2 can be used as independent factors for the prognosis of OSCC patients. Finally, our FISH detection results for MDM2 showed that the high expression of MDM2 was significantly correlated with the amplification of MDM2 (p=0.015). This study shows that MDM2 and p53 can be used as independent predictors of the prognosis of patients with oesophageal squamous cell carcinoma.