Human autoantibodies to the thyrotropin receptor: recognition of linear, folded, and glycosylated recombinant extracellular domain.
Human autoantibodies to the thyrotropin receptor: recognition of linear, folded, and glycosylated recombinant extracellular domain.
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促甲状腺素受体的人类自身抗体：线性、折叠和糖基化重组胞外结构域的识别。
DOI：
10.1210/jcem.80.1.7829638
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
T. Davies
中科院分区：
文献类型：
--
作者：
H. Vlase;P. Graves;R. Magnusson;T. Davies
为了检测抗人促甲状腺激素受体（hTSHR）自身抗体的异质性，我们评估了20例Graves病患者血清对原核（非糖基化）和真核（昆虫细胞糖基化）重组hTSHR胞外结构域（ecd）在未折叠（线性）和折叠（非线性）状态下的识别。与原核抗原，12（60%）结合折叠的hTSHR ecd单体，8（40%）结合的未折叠的单体，和3（15%）结合的四聚体物质。这种与不同hTSHR抗原的结合不是相互排斥的。此外，7份（35%）血清对折叠单体的反应性明显高于未折叠单体。当与糖基化的昆虫细胞hTSHR ecd反应时，9个（45%）血清识别未折叠和折叠的单体，5个（25%）识别四聚体形式。在我们的所有测试中，20份血清中的17份（85%）结合1种或多种重组hTSHR ecd抗原，并且在至少4份（20%）血清样品中的识别模式似乎是异质的，hTSHR抗体识别线性、折叠和糖基化的hTSHR ecd单体。因此，我们的结论是，Graves病患者的自身抗体，识别多个表位的hTSHR ecd和它是可能的，以分类，根据他们的识别线性，折叠，和糖基化的产品。
To examine the heterogeneity of autoantibodies to the human TSH receptor (hTSHR), we evaluated 20 sera from patients with Graves' disease for their recognition of prokaryotic (unglycosylated) and eukaryotic (insect cell glycosylated) recombinant hTSHR extracellular domain (ecd) in an unfolded (linear) and a folded (nonlinear) state. With the prokaryotic antigen, 12 (60%) bound folded hTSHR ecd monomer, 8 (40%) bound to the unfolded monomer, and 3 (15%) bound to a tetrameric species. Such binding to different hTSHR antigens was not mutually exclusive. In addition, 7 (35%) sera showed an apparently higher reactivity for the folded than the unfolded monomer. When reacted against the glycosylated insect cell hTSHR ecd, 9 (45%) sera recognized both the unfolded and folded monomer, and 5 (25%) recognized the tetrameric form. In all of our testing, 17 of the 20 sera (85%) bound to 1 or more of the recombinant hTSHR ecd antigens, and the recognition pattern appeared to be heterogeneous in at least 4 (20%) of the serum samples, with hTSHR antibodies recognizing linear, folded, and glycosylated hTSHR ecd monomers. We conclude, therefore, that patients with Graves' disease have autoantibodies that recognize multiple epitopes on the hTSHR ecd and that it is possible to classify them according to their recognition of linear, folded, and glycosylated products.
使用人促甲状腺素受体的重组胞外结构域和合成肽分析格雷夫斯病患者的自身抗体反应性。
影响因子：
3.5
作者：
Fan,JL;Seetharamaiah,GS;Desai,RK;Dallas,JS;Wagle,NM;Prabhakar,BS
通讯作者：
Prabhakar,BS
影响因子：
2.9
作者：
HAGER, DA;BURGESS, RR
通讯作者：
BURGESS, RR