Dentin sialophosphoprotein-promoted mineralization and expression of odontogenic genes in adipose-derived stromal cells
Dentin sialophosphoprotein-promoted mineralization and expression of odontogenic genes in adipose-derived stromal cells
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DOI：
10.1159/000110079
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发表时间：
2008-01-01
影响因子：
2.7
通讯作者：
Tian, Weidong
中科院分区：
文献类型：
--
作者：
Wu, Ling;Zhu, Feng;Tian, Weidong
牙本质涎磷蛋白（DSPP）是一种细胞外基质，是典型的牙本质和骨特异性基因，在牙本质矿化和牙齿发育中起重要作用。脂肪源性基质细胞（ADSCs）被认为含有一组多能间充质干细胞，其能够在体外或体内矿化。在本研究中，我们假设过表达DSPP会促进ADSC的矿化。结果表明，表达DSPP的腺病毒（Ad-DSPP）感染ADSCs后，可促进矿化相关基因Cbfa1、Osx、BSP、OCN和DMP1的表达。细胞化学和碱性磷酸酶活性测定也证实了Ad-DSPP感染的ADSCs中碱性磷酸酶活性。矿化实验表明Ad-DSPP感染的ADSCs能够形成矿化结节。本研究的另一个发现是，早期牙源性标记基因如Msx1，Msx2，Lhx7和Pax9在DSPP过表达的ADSC中表达。因此，我们的研究结果表明，DSPP的过度表达促进了ADSCs的矿化，并与早期牙源性标记基因的表达一起，暗示这些细胞可能分化为功能性成牙本质细胞样细胞。版权所有（C）2007 S. Karger AG，巴塞尔。
Dentin sialophosphoprotein (DSPP) is an extracellular matrix, typically dentin- and bone-specific gene, which plays an important role in dentin mineralization and tooth development. Adipose-derived stromal cells (ADSCs) are considered to contain a group of pluripotent mesenchymal stem cells which are capable of mineralization either in vitro or in vivo. In the present study, we hypothesized that overexpression of DSPP would promote mineralization in ADSCs. Our results showed that infection of DSPP-expressing adenovirus (Ad-DSPP) enhanced expression of genes related to mineralization, such as Cbfa1, Osx, BSP, OCN and DMP1 in ADSCs. Alkaline phosphatase activity was also confirmed in Ad-DSPP-infected ADSCs by cytochemistry and alkaline phosphatase activity assay. Mineralization assay indicated that Ad-DSPP-infected ADSCs were able to form mineralized nodules. Another finding in this study is that early odontogenic marker genes such as Msx1, Msx2, Lhx7 and Pax9 were expressed in DSPP-overexpressed ADSCs. Thus, our results suggested that overexpression of DSPP promoted mineralization of ADSCs, and together with the expression of early odontogenic marker genes, implied that these cells may differentiate into functional odontoblast-like cells. Copyright (C) 2007 S. Karger AG, Basel.