Analysis of microbiota in elderly patients with Acute Cerebral Infarction
Analysis of microbiota in elderly patients with Acute Cerebral Infarction
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作者：
Huang, Lin;Wang, Teng;Wan, Qi
背景和目标。最近的证据表明，微生物群与几乎所有主要类型的疾病有关，包括心血管疾病。然而，其在急性脑梗死中的作用仍有待探讨。了解急性脑梗死患者肠道微生物群（GM）的多样性和分布，以及GM在这类疾病中的作用具有重要意义。我们对40名患者的肠道微生物群进行了焦磷酸测序，以阐明急性脑梗死患者和健康对照者之间的微生物群组成是否不同：在这些患者中，有31名急性脑梗死患者和9名对照者。我们应用线性回归计算肠道植物群与疾病危险因素之间的相关性。最后，通过KEGG功能富集分析，探讨肠道植物群与急性脑梗死的相关性。对照组和患者组的总体微生物结构相似，但对照组中存在较高的布劳特氏菌，而患者组中存在较高的链球菌和肺炎普雷沃氏菌。线性回归分析发现，布劳特氏菌与白色血细胞计数呈负相关，链球菌与肌酐和脂蛋白呈正相关。KEGG通路分析表明，包括甲烷代谢，脂多糖合成，细菌分泌和鞭毛组装的肠道微生物群在患者组中的生物通路的表达与对照组不同。我们发现了急性脑梗死患者肠道微生物功能的3种差异表达，并发现了4种可能与急性脑梗死发病相关的细菌途径。我们的研究确定了三种异常表达的细菌-它揭示了这些细菌物种与急性脑梗死的相关性，因为它们与疾病因素和功能途径有关。这些发现可能有助于脑梗死的治疗，因为肠道微生物群可以作为治疗心血管和代谢疾病的潜在治疗方法。
Background and Aims. Recent evidence suggest that microbiota is associated with almost all major types of diseases, including cardiovascular diseases. However, its role in Acute Cerebral Infarction remains unexplored. It is important to understand the diversity and distribution of gut microbiota (GM) in patients with Acute Cerebral Infarction and the role that GM plays in this type of disease.Methods. We performed pyrosequencing on the gut microbiota of 40 individuals in order to elucidate whether the composition of the microbiota differs between patients with Acute Cerebral Infarction and healthy controls: Of these individuals, there were 31 with Acute Cerebral Infarction and nine controls. We applied linear regression to calculate the correlation between the gut flora and disease risk factors. Finally, KEGG functional enrichment analysis was conducted to examine the correlation between the gut flora and Acute Cerebral Infarction.Results. The overall microbial structure was similar in both the controls and the patients, but the control group had higher relative presence of Blautia obeum while the presence of Streptococcus infantis and Prevotella copri were relatively higher in the patient group. Using linear regression, we found that Blautia obeum was negatively associated with white blood cell count and Streptococcus infantis was positively correlated with creatinine and lipoprotein. The KEGG pathway analysis indicated that the bio-pathways including methane metabolism, lipopolysaccharide synthesis, bacterial secretion, and flagellar assembly of the gut microbiota in the patient group was expressed differently than that of the controls. We identified three differentially expressed gut microbial functions in Acute Cerebral Infarction and found four bacterial pathways that might be related to the development of this disease.Conclusions. Our study identified three abnormally-expressed bacteria-Blautia obeum, Streptococcus infantis, and Prevotella copri-in patients with Acute Cerebral Infarction compared with healthy controls. It reveals a correlation of these bacterial species with Acute Cerebral Infarction as they relate to disease factors and functional pathways. These findings may shed light on the treatment of cerebral infarction because gut microbiota could serve as a potential therapeutic approach for the treatment of cardiovascular and metabolic diseases.