Ultra high-speed sorting
Ultra high-speed sorting
复制标题
DOI：
10.1002/cyto.a.20160
复制
发表时间：
2005-10-01
期刊：
影响因子：
3.7
通讯作者：
Leary, JF
中科院分区：
文献类型：
--
作者：
Leary, JF
背景：细胞分选的历史可以追溯到大约40年前。主要的局限性在于，虽然流式细胞术是一门科学，但在大多数时间里细胞分选一直是一门艺术。最近的进展，协助技术有助于减少量的专业知识，必要的执行sorting.Methods：液滴为基础的分拣是基于一个受控的扰动射流依赖于表面张力。分选产率和纯度高度依赖于稳定的射流断开位置。系统压力和孔直径决定了每秒的液滴数量，这是分选速率的限制步骤，因为现代电子设备可以处理更高的细胞信号处理率。复杂的细胞分选还需要新的和更复杂的分选决策，特别是当细胞亚群是罕见的，需要从后面提取-高速分选继续造成重大问题，由于产生的气溶胶的生物安全性。结论：细胞分选已变得更加稳定和可预测的，需要较少的专业知识来操作。然而，气溶胶遏制的问题继续使得基于液滴的细胞分选成问题。流体物理学和细胞活力限制对几乎已经达到的高速分选提出了实际限制。在接下来的5年里，芯片实验室微流体系统中的流体切换分选可能会取得进展，不仅可以解决气溶胶和生存力问题，而且还可以通过大规模并行和指数分级微流体架构实现超高速分选。(c)2005年国际分析细胞学学会。
Background: Cell sorting has a history dating back approximately 40 years. The main limitation has been that, although flow cytometry is a science, cell sorting has been an art during most of this time. Recent advances in assisting technologies have helped to decrease the amount of expertise necessary to perform sorting.Methods: Droplet-based sorting is based on a controlled disturbance of a jet stream dependent on surface tension. Sorting yield and purity are highly dependent on stable jet break-off position. System pressures and orifice diameters dictate the number of droplets per second, which is the sort rate limiting step because modern electronics can more than handle the higher cell signal processing rates.Results: Cell sorting still requires considerable expertise. Complex multicolor sorting also requires new and more sophisticated sort decisions, especially when cell subpopulations are rare and need to be extracted from back- High-speed sorting continues to pose major problems in terms of biosafcty due to the aerosols generated.Conclusions: Cell sorting has become more stable and predictable and requires less expertise to operate. However, the problems of aerosol containment continue to make droplet-based cell sorting problematical. Fluid physics and cell viability restraints pose practical limits for high-speed sorting that have almost been reached. Over the next 5 years there may be advances in fluidic switching sorting in lab-on-a-chip microfluidic systems that could not only solve the aerosol and viability problems but also make ultra high-speed sorting possible and practical through massively parallel and exponential staging microfluidic architectures. (c) 2005 International Society for Analytical Cytology.