AGE-RELATED-CHANGES IN MUCINS FROM HUMAN WHOLE SALIVA
AGE-RELATED-CHANGES IN MUCINS FROM HUMAN WHOLE SALIVA
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DOI：
10.1177/00220345910700100201
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发表时间：
1991-10-01
影响因子：
7.6
通讯作者：
TABAK, LA
中科院分区：
文献类型：
--
作者：
DENNY, PC;DENNY, PA;TABAK, LA
人全唾液中的主要粘蛋白MG 1和MG 2用于保护和润滑口腔。在这项研究中，从两组受试者中收集未刺激和刺激的全部唾液：年轻人（18 - 35岁）和老年人（65 - 83岁）。受试者的健康状况明显良好。通过SDS-PAGE分析每位受试者的唾液样本。凝胶用双抗染色，并通过视频图像密度测定法定量MG 1和MG 2。该方案给出了每种粘蛋白的可重现值。从老年受试者的刺激和未刺激的唾液显示出显着减少的浓度的MG 1和MG 2，定量粘蛋白染料结合单位。这些减少与老化过程的可能关联进行了讨论。
The predominant mucins in human whole saliva, MG1 and MG2, serve to protect and to lubricate the oral cavity. In this study, both unstimulated and stimulated whole salivas were collected from two groups of subjects: young (18-35 years of age) and aged (65-83 years of age). The subjects were in apparent good health. Saliva samples from each subject were analyzed by SDS-PAGE. The gels were stained with Stains-all, and both MG1 and MG2 were quantitated by video-image densitometry. The protocol gave reproducible values for each mucin. The stimulated and unstimulated salivas from aged subjects showed significant reductions in concentrations of both MG1 and MG2, as quantitated in mucin dye-binding units. Possible associations of these reductions with the aging process are discussed.