Comprehensive analysis of the homeodomain-leucine zipper IV transcription factor family in Cucumis sativus
Comprehensive analysis of the homeodomain-leucine zipper IV transcription factor family in Cucumis sativus
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黄瓜同源结构域-亮氨酸拉链IV转录因子家族的综合分析
DOI：
10.1139/gen-2013-0143
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发表时间：
2013-07-01
期刊：
影响因子：
3.1
通讯作者：
Ren, Zhonghai
中科院分区：
文献类型：
--
作者：
Fu, Rao;Liu, Wei;Ren, Zhonghai
IV类同源结构域亮氨酸拉链（HD-Zip IV）蛋白是植物特异性转录因子，已知在植物生长和发育中起关键作用。在这项研究中，11个黄瓜（黄瓜）HD-Zip IV基因在版本2黄瓜基因组中被鉴定，并发现在染色体上分布不均匀。CsHDZIV（黄瓜同源结构域亮氨酸拉链IV）基因家族比其他研究物种（水稻除外）小，因为没有基因重复事件。系统进化分析表明，黄瓜、拟南芥、番茄、棉花、玉米和水稻的HD-Zip IV基因可分为5个亚类。所有的CsHDZIV基因似乎都来自于编码区中含有11个外显子的基本模块。在6个CsHDZIV基因的3 '端非翻译区发现了21和19个核苷酸的保守基序，提示转录后调控可能在CsHDZIV基因的调控中发挥作用。此外，发现11个CsHDZIV基因中的6个经历选择性剪接事件。反转录PCR分析表明，所有CsHDZIV基因（除了一个）的表达，并显示在生殖器官中的优先表达。
The class IV homeodomain-leucine zipper (HD-Zip IV) proteins are plant-specific transcriptional factors known to play crucial roles in plant growth and development. In this study, 11 cucumber (Cucumis sativus) HD-Zip IV genes were identified in the version 2 cucumber genome and found to be distributed unevenly across the chromosomes. The CsHDZIV (Cucumis sativus homeodomain-leucine zipper IV) gene family is smaller than in other studied species (except for rice) because of the absence of gene duplication events. Phylogenetic analysis showed that HD-Zip IV genes from cucumber, Arabidopsis, tomato, cotton, maize, and rice could be classified into five subgroups. All CsHDZIV genes appear to be derived from a basic module containing 11 exons in the coding region. Two conserved motifs of 21 and 19 nucleotides were found in the 3'-untranslated regions of six CsHDZIV genes, suggesting that post-transcriptional regulation may play a role in regulation of CsHDZIV genes. In addition, 6 of 11 CsHDZIV genes were found to undergo alternative splicing events. Reverse transcription PCR analysis showed that all CsHDZIV genes (except one) were expressed and showed preferential expression in reproductive organs.