Poly-lactic-glycolic-acid surface nanotopographies selectively decrease breast adenocarcinoma cell functions.
Poly-lactic-glycolic-acid surface nanotopographies selectively decrease breast adenocarcinoma cell functions.
复制标题
聚乳酸乙醇酸表面纳米拓扑结构选择性地降低乳腺腺癌细胞功能。
DOI：
10.1088/0957-4484/23/15/155101
复制
发表时间：
2012
期刊：
影响因子：
3.5
通讯作者：
Webster,ThomasJ
中科院分区：
文献类型：
--
作者：
Zhang,Lijuan;Webster,ThomasJ
聚乳酸-羟基乙酸（PLGA, 50:50 PLG/PGA, wt%）纳米形貌降低肺上皮癌细胞功能（包括粘附、增殖、凋亡和血管内皮生长因子（VEGF）分泌）的能力此前已有报道。具体来说，结果表明，与传统的纳米光滑PLGA相比，23 nm表面特征PLGA上肺上皮癌细胞VEGF合成减少。然而，很明显，不同的细胞系在相似的生物材料上可能有不同的行为。因此，为了研究纳米化PLGA底物抑制多种癌细胞功能的普遍性，我们在不同的PLGA纳米表面形貌上检测了乳腺上皮腺癌细胞（MCF-7）的粘附、增殖、凋亡和VEGF分泌。为了将表面纳米形貌效应与所有其他表面性质分离开来，在这里制备了具有不同纳米形貌但化学性质和疏水性相似的PLGA表面。原子力显微镜（AFM）验证了本研究制备的PLGA表面的不同纳米形貌。重要的是，研究结果首次表明，与其他所有PLGA表面形貌（特别是纳米光滑、300和400 nm表面特征的PLGA表面）相比，23 nm表面特征的PLGA表面显著降低了乳腺腺癌细胞的功能（包括增殖率降低、凋亡增加和VEGF合成减少）。相比之下，与所有其他PLGA样本相比，健康乳腺上皮细胞在23 nm的PLGA表面上增殖更多（24%）。总之，这些结果为理解PLGA表面纳米形貌对癌细胞功能的作用提供了进一步的见解，更重要的是，开辟了将聚合物纳米形貌用于广泛的抗癌再生医学应用的可能性（不需要使用化疗药物）。
The ability of poly (lactic-co-glycolic acid)(PLGA, 50: 50 PLG/PGA, wt%) nanotopographies to decrease lung epithelial carcinoma cell functions (including adhesion, proliferation, apoptosis and vascular endothelial growth factor (VEGF) secretion) has been previously reported. Specifically, results demonstrated decreased lung epithelial carcinoma cell VEGF synthesis on 23 nm surface-featured PLGA compared to traditional nanosmooth PLGA. However, clearly, different cell lines could have different behaviors on similar biomaterials. Thus, to investigate the universality of nanopatterned PLGA substrates to inhibit numerous cancer cell functions, here, breast epithelial adenocarcinoma cell (MCF-7) adhesion, proliferation, apoptosis and VEGF secretion were determined on different PLGA nanometer surface topographies. To isolate surface nanotopographical effects from all other surface properties, PLGA surfaces with various nanotopographies but similar chemistry and hydrophobicity were fabricated here. Atomic force microscopy (AFM) verified the varied nanotopographies on the PLGA surfaces prepared in this study. Importantly, results demonstrated for the first time significantly decreased breast adenocarcinoma cell functions (including decreased proliferation rate, increased apoptosis and decreased VEGF synthesis) on 23 nm featured PLGA surfaces compared to all other PLGA surface topographies fabricated (specifically, nanosmooth, 300 and 400 nm surface-featured PLGA surfaces). In contrast, healthy breast epithelial cells proliferated more (24%) on the 23 nm featured PLGA surfaces compared to all other PLGA samples. In summary, these results provided further insights into understanding the role PLGA surface nanotopographies can have on cancer cell functions and, more importantly, open the possibility of using polymer nanotopographies for a wide range of anticancer regenerative medicine applications (without resorting to the use of chemotherapeutics).