Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts
Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts
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DOI：
10.1016/j.exphem.2007.12.015
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发表时间：
2008-05-01
影响因子：
2.6
通讯作者：
Zago, Marco A.
中科院分区：
文献类型：
--
作者：
Covas, Dimas T.;Panepucci, Rodrigo A.;Zago, Marco A.
Objective.多能间充质基质细胞（MSC）与周细胞和成纤维细胞的关系迄今尚未建立，尽管它们共享原始骨髓基质细胞的许多标志物以及成骨、成脂和成软骨分化潜能。我们比较了来自成人或胎儿组织的MSC、体外分化的MSC、成纤维细胞和通过抗CD 146分离或粘附获得的视网膜周细胞培养物。其特征包括形态学、免疫表型、基因表达谱和分化潜能。MSC、视网膜血管周围细胞和成纤维细胞的成骨、脂肪细胞和软骨细胞分化被证明。细胞形态和22个标记物定义的表型非常相似。通过对17个文库的基因表达进行系列分析和对来自31个不同细胞培养物的39个选定基因进行逆转录聚合酶链反应，对获得的全局基因表达进行分析，揭示了MSC、视网膜血管周围细胞和肝星状细胞之间的相似性。尽管这种总体相似性，有一个与血管生成相关的基因的异质性表达，在MSC来自静脉，动脉，血管周围细胞，和成纤维细胞。通过实时聚合酶链反应评价典型的周细胞和MSC转录物，如CD 146选择的血管周围细胞和MSC上的NG 2、CD 146、CD 271和CD 140 B，证实了这两种细胞类型之间的关系。此外，在周细胞、MSC和成纤维细胞上观察到的成纤维细胞特异性蛋白-1和CD 146转录物之间的负相关性突出了它们作为该分化途径的标志物的潜在用途。我们的研究结果表明，人MSC和周细胞是位于血管壁中的相似细胞，在那里它们作为修复和组织维护的细胞来源发挥作用，而成纤维细胞是分化程度更高的细胞，分化潜力更有限。(C)2008 ISEH -血液学和干细胞学会。爱思唯尔公司出版
Objective. The relationship of multipotent mesenchymal stromal cells (MSC) with pericytes and fibroblasts has not been established thus far, although they share many markers of primitive marrow stromal cells and the osteogenic, adipogenic, and chondrogenic differentiation potentials.Materials and Methods. We compared MSCs from adult or fetal tissues, MSC differentiated in vitro, fibroblasts and cultures of retinal pericytes obtained either by separation with anti-CD146 or adhesion. The characterizations included morphological, immunophenotypic, gene-expression profile, and differentiation potential.Results. Osteogenic, adipocytic, and chondrocytic differentiation was demonstrated for MSC, retinal perivascular cells, and fibroblasts. Cell morphology and the phenotypes defined by 22 markers were very similar. Analysis of the global gene expression obtained by serial analysis of gene expression for 17 libraries and by reverse transcription polymerase chain reaction of 39 selected genes from 31 different cell cultures, revealed similarities among MSC, retinal perivascular cells, and hepatic stellate cells. Despite this overall similarity, there was a heterogeneous expression of genes related to angiogenesis, in MSC derived from veins, artery, perivascular cells, and fibroblasts. Evaluation of typical pericyte and MSC transcripts, such as NG2, CD146, CD271, and CD140B on CD146 selected perivascular cells and MSC by real-time polymerase chain reaction confirm the relationship between these two cell types. Furthermore, the inverse correlation between fibroblast-specific protein-1 and CD146 transcripts observed on pericytes, MSC, and fibroblasts highlight their potential use as markers of this differentiation pathway.Conclusion. Our results indicate that human MSC and pericytes are similar cells located in the wall of the vasculature, where they function as cell sources for repair and tissue maintenance, whereas fibroblasts are more differentiated cells with more restricted differentiation potential. (C) 2008 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.