Vitellogenin regulates antimicrobial responses in Chinese mitten crab, Eriocheir sinensis
Vitellogenin regulates antimicrobial responses in Chinese mitten crab, Eriocheir sinensis
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卵黄蛋白原调节中华绒螯蟹的抗菌反应
DOI：
10.1016/j.fsi.2017.08.002
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发表时间：
2017-10-01
影响因子：
4.7
通讯作者：
Wang, Qun
中科院分区：
文献类型：
--
作者：
Li, Lu;Li, Xue Jie;Wang, Qun
卵黄蛋白原(VTG)传统上被认为是胚胎和幼虫早期发育过程中营养和能量的主要供应者，但越来越多的证据表明VTG也参与了先天性免疫防御。VTG是否参与中华绒螯蟹的先天免疫，其功能在很大程度上尚不清楚。本研究克隆了中华绒毛虫卵黄蛋白原1基因(Es-vtg1)。该基因全长为7939个核苷酸，编码2567个氨基酸残基的开放阅读框。生物信息学分析表明，ES-Vtg1的结构域在进化过程中是保守的。实时荧光定量聚合酶链式反应和免疫印迹结果显示ES-VTG1在卵巢和肝胰腺中均有高表达。此外，细菌可诱导ES-Vtg1的高水平表达。ES-Vtg1在免疫防御中发挥重要作用，包括与细菌结合、抑制细菌增殖、调节抗菌肽的表达等。综上所述，这些结果表明ES-Vtg1在抗菌功能中起着关键作用。(C)2017爱思唯尔有限公司。保留所有权利。
Vitellogenin (Vtg) is traditionally regarded as a key supplier of nutrients and energy during the early development of embryos and larvae, but accumulating evidence suggests that Vtg is also involved in innate immune defense. Whether Vtg is involved in innate immunity in Eriocheir sinensis, and its functions, remain largely unknown. In this study, a cDNA representing the vitellogenin1 gene from E. sinensis (Es-vtg1) was cloned. The full-length Es-vtg1 cDNA comprised 7939 nucleotides, encoding an open-reading frame of 2567 amino acid residues. Bioinformatic analysis showed that the domains of Es-Vtg1 have been conserved during evolution. Quantitative real-time PCR and western blotting showed that Es-vtg1 was highly expressed in ovary and hepatopancreas. Moreover, bacteria could induce the high-level expression of Es-Vtg1. Es-Vtg1 plays important roles in immunological defense, including binding to bacteria, inhibiting bacterial proliferation, and regulating the expression of antimicrobial peptides. Collectively, these results demonstrate that Es-Vtg1 plays critical roles in antimicrobial function. (C) 2017 Elsevier Ltd. All rights reserved.