COMPARISON OF THE SENSITIVITY OF THE VAGINAL MUCOUS-MEMBRANES OF THE ALBINO RABBIT AND LABORATORY RAT TO NONOXYNOL-9
COMPARISON OF THE SENSITIVITY OF THE VAGINAL MUCOUS-MEMBRANES OF THE ALBINO RABBIT AND LABORATORY RAT TO NONOXYNOL-9
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DOI：
10.1016/0278-6915(85)90161-9
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发表时间：
1985-01-01
影响因子：
4.3
通讯作者：
WILLIGAN, DA
中科院分区：
文献类型：
--
作者：
KAMINSKY, M;SZIVOS, MM;WILLIGAN, DA
进行了研究，比较了白化病兔和白化病大鼠阴道粘膜对表面活性剂壬苯聚醇-9刺激的敏感性。该比较的目的是评价大鼠作为筛选阴道产品潜在刺激性的模型。通过灌洗阴道内给予不同浓度的壬苯醇醚-9的蒸馏水溶液，每天一次，连续4天。在第5天，处死动物，处理阴道组织并进行组织病理学检查。使用预先确定的半定量评分系统，发现刺激强度在两个种属中均具有浓度依赖性。在家兔中，刺激性显著大于大鼠，浓度为2.5%和5%时为轻度，浓度为12.5%和25%时为中度至重度。在大鼠中，刺激性可忽略不计，高达12.5%，在25%时变为轻度，在75%时变为中度。因此，家兔的阴道粘膜比大鼠的阴道粘膜更敏感，并且可能比大鼠粘膜更能夸大化合物对人阴道的刺激潜力。
Studies were conducted to compare the sensitivities of the vaginal mucous membranes of the albino rabbit and albino rat to irritation by the surfactant nonoxynol-9. The purpose of this comparison was to evaluate the rat as a model for screening vaginal products for their potential irritancy. Nonoxynol-9 at various concentrations in distilled water was administered intravaginally by lavage once a day for four consecutive days. On day 5, the animals were killed and the vaginal tissues were processed and examined histopathologically. Using a predetermined semiquantitative scoring system, it was found that the intensity of irritation was concentration dependent in both species. In the rabbit, the irritation was significantly greater than that in the rat, being mild with concentrations of 2.5 and 5% and moderate to severe at 12.5 and 25%. In the rat, irritation was negligible up to 12.5%, becoming mild at 25% and more moderate at 75%. Thus the vaginal mucosa of the rabbit is more sensitive than that of the rat and may be expected to give a more exaggerated representation of the irritation potential of a compound to the human vagina than would the rat mucosa.