A truncated CC-NB-ARC gene TaRPP13L1-3D positively regulates powdery mildew resistance in wheat via the RanGAP-WPP complex-mediated nucleocytoplasmic shuttle
A truncated CC-NB-ARC gene TaRPP13L1-3D positively regulates powdery mildew resistance in wheat via the RanGAP-WPP complex-mediated nucleocytoplasmic shuttle
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DOI：
10.1007/s00425-022-03843-0
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发表时间：
2022-03-01
期刊：
影响因子：
4.3
通讯作者：
Ji，Wanquan
中科院分区：
文献类型：
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作者：
Zhang，Xiangyu;Wang，Guanghao;Ji，Wanquan
主要结论小麦RPP 13-like亚型与WPP 1相互作用，有助于对白粉病（Blumeria graminisf.摘要植物抗病基因（R）赋予植物抵抗病原菌侵染的能力，而病原菌表达特异的无毒基因。霜霉13-like（RPP 13-like）基因属于NBS-LRR超家族，在植物抗病过程中发挥重要作用。之前，我们检测到TaRPP 1 - 3样基因位于染色体3D（TaRPP 1 - 3L 1 -3D）的TaSpl 1驻留区域，其被细胞死亡表型强烈诱导（Zhang et al. 2021）。TaRPP 1 - 3 L1 - 3D基因编码一个典型的NB-ARC结构，具有Rx-N和P-loop NTR结构域; TaRPP 1 - 3 L1 - 3D基因转录本在小麦白粉病（Blumeria graminisf.）小麦条锈菌（Pucciniastriiformisf.）尽管在小麦-Bgtin不相容和相容背景下观察到相反的表达模式。TaRPP 1 - 3 L1 - 3D的过表达增强了小麦对Bgt的抗病性，同时上调了防御相关标记基因PAD 4、类奇异果甜蛋白（TLP）和类几丁质酶8蛋白（Chi 8L）的表达，而TaRPP 1 - 3 L1 - 3D的沉默则破坏了小麦对Bgt的抗病性。亚细胞定位研究表明，TaRPP 1 - 3 L1 -3D位于质膜和细胞核中，而酵母双杂交（Y2 H）试验表明，TaRPP 1 - 3 L1 -3D与WPP结构域蛋白1（TaWPP 1）相互作用。这表明TaRPP 1 - 3 L1 -3D通过由核孔中的RanGAP-WPP复合物介导的机制在核和细胞质膜之间穿梭。对TaRPP 1 - 3L 1 - 3D的深入研究将有助于揭示小麦抗真菌的机制，以及RPP 1 - 3L 1 - 3D基因表达与病原菌防御之间的相互作用。
Main ConclusionA wheat RPP13-like isoform interacting with WPP1 contributes to quantitative and/or basal resistance to powdery mildew (Blumeria graminisf. sp.tritici) by restricting the development ofBgtconidia.AbstractPlant disease resistance (R) genes confer an ability to resist infection by pathogens expressing specific avirulence genes. Recognition ofPeronospora parasitica13-like (RPP13-like) genes belong to the nucleotide-binding site and leucine-rich repeat (NBS-LRR) superfamily and play important roles in resistance to various plant diseases. Previously, we detected aTaRPP13-like gene located on chromosome 3D (TaRPP13L1-3D) in theTaSpl1resided region, which is strongly induced by the cell death phenotype (Zhang et al. 2021). Here, we investigated the expression and functional role ofTaRPP13L1-3Din wheat responding to fungal stress.TaRPP13L1-3Dencoded a typical NB-ARC structure characterized by Rx-N and P-loop NTPase domains.TaRPP13L1-3Dtranscripts were strongly upregulated in wheat by powdery mildew (Blumeria graminisf. sp.tritici;Bgt) and stripe rust (Puccinia striiformisf. sp.tritici;Pst) infection although opposing expression patterns were observed in response to wheat-Bgtin incompatible and compatible backgrounds. Overexpression ofTaRPP13L1-3Denhanced disease resistance toBgt, accompanied by upregulation of the defense-related marker genes encoding phytoalexin-deficient4 (PAD4), thaumatin-like protein (TLP) and chitinase 8-like protein (Chi8L), while silencing ofTaRPP13L1-3Ddisrupted the resistance toBgtinfection. Subcellular localization studies showed that TaRPP13L1-3D is located in both the plasma membrane and nucleus, while yeast-two-hybrid (Y2H) assays indicated that TaRPP13L1-3D interacts with WPP domain-containing protein 1 (TaWPP1). This indicates that TaRPP13L1-3D shuttles between the nucleus and cytoplasm membrane via a mechanism that is mediated by the RanGAP-WPP complex in nuclear pores. This insight intoTaRPP13L1-3Dwill be useful in dissecting the mechanism of fungal resistance in wheat, and understanding the interaction betweenRgene expression and pathogen defense.