The mechanism through which dietary supplementation with heated linseed grain increases n-3 long-chain polyunsaturated fatty acid concentration in subcutaneous adipose tissue of cashmere kids
The mechanism through which dietary supplementation with heated linseed grain increases n-3 long-chain polyunsaturated fatty acid concentration in subcutaneous adipose tissue of cashmere kids
复制标题
膳食中添加加热亚麻籽粒增加羊绒山羊皮下脂肪组织中n-3长链多不饱和脂肪酸浓度的机制
DOI：
10.1093/jas/sky386
复制
发表时间：
2019
影响因子：
3.3
通讯作者：
Yan Sumei
中科院分区：
文献类型：
--
作者：
Wang Xue;Martin Graeme B;Liu Shulin;Shi Binlin;Guo Xiaoyu;Zhao Yanli;Yan Sumei
本研究旨在探讨日粮中添加加热亚麻籽对阿尔巴斯白色绒山羊血浆、肝脏和皮下脂肪组织（SADT）脂肪酸（FA）组成的影响，特别是对SADT中n-3长链多不饱和脂肪酸谱和脂质代谢相关基因mRNA表达的影响。选择4月龄去势公羔60只，平均体重18.6 ± 0.1kg，采用随机区组设计，随机分为3组。使用三种饮食处理：（1）不添加补充剂的基础饮食（对照），（2）添加亚麻籽油（LSO）的基础饮食，和（3）添加加热的亚麻籽谷物（HLS）的基础饮食。试验期为104 d，其中适应期14 d，测定期90 d。LSO和HLS的SADT FA谱不同。与LSO组相比，HLS组的C18：3 n3（P< 0.0001）、C22：6 n3（P= 0.007）和n-3 PUFA（P< 0.0001）含量较高，n-6/n-3比值较低（P< 0.0001）。LSO和HLS儿童之间的FA差异是由于HLS儿童SADT中极长链FA蛋白5（P< 0.0001）、δ-6去饱和酶（P< 0.0001）和过氧化物酶体增殖物激活受体α（P= 0.003）的表达增加，并且还与肝脏脂肪代谢有关。总之，这些结果表明，在SADT中，HLS的消耗通过增加肝脏C22：6 n3含量和通过促进PPARα表达增加SADT中的C22：6 n3和FADS 2的mRNA表达而导致比LSO更多的C22：6 n3。
The aim of this study was to investigate the effects of dietary supplementation with heated linseed on the fatty acid(FA)composition of the plasma, liver, and subcutaneous adipose tissue(SADT)of Albas white cashmere kids, particularly the effect on n-3 long-chain polyunsaturated FA profiles and the mRNA expression of genes related to lipid metabolism in SADT. Sixty 4-month-old castrated male kids (average BW 18.6 ± 0.1 kg) were selected and randomly allocated into three groups in a randomized block design. Three dietary treatments were used: (1) basal diet without supplementation (Control), (2) basal diet supplemented with linseed oil(LSO), and (3) basal diet supplemented with heated linseed grain(HLS). The diets were fed for 104 d, consisting of 14 d for adaptation followed by 90 d of measurement. Different FA profiles were found in SADT between LSO and HLS. Kids fed HLS had more C18:3n3 (P< 0.0001), C22:6n3 (P= 0.007), and n-3 PUFA (P< 0.0001) and a less (P< 0.0001) n-6/n-3 ratio than LSO kids. These FA differences between LSO and HLS kids were due to the increased expression of elongation of very long chain FA protein 5 (P< 0.0001), delta-6 desaturase (P< 0.0001), and peroxisome proliferator-activated receptor α (P= 0.003) in SADT of HLS kids and was also associated with liver fat metabolism. Together, these results suggest that the consumption of HLS leads to more C22:6n3 than LSO in SADT by increasing liver C22:6n3 content and by increasing SADT mRNA expression of ELOVL5 and FADS2 through promoting PPARα expression.