Single cell full-length transcriptome of human subcutaneous adipose tissue reveals unique and heterogeneous cell populations.
Single cell full-length transcriptome of human subcutaneous adipose tissue reveals unique and heterogeneous cell populations.
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DOI：
10.1016/j.isci.2022.104772
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发表时间：
2022-08-19
期刊：
影响因子：
5.8
通讯作者：
Sparks, Lauren M
中科院分区：
文献类型：
--
作者：
Whytock, Katie L;Sun, Yifei;Divoux, Adeline;Yu, GongXin;Smith, Steven R;Walsh, Martin J;Sparks, Lauren M
白色脂肪组织是脂肪细胞和非成脂细胞的复合体。表征WAT的细胞组成对于确定影响新陈代谢的潜在变化发生在哪里至关重要。大多数单细胞(Sc)RNA-Seq研究集中在不包含脂肪细胞的间质血管部分(SVF)，并使用了具有3‘或5’偏向的技术。利用全长sc/单核(Sn)RNA-Seq技术，我们利用整体组织的SnRNA-Seq、分离脂肪细胞的SnRNA-Seq和SVF的scRNA-Seq来查询WAT的转录组成。整个Wat SnRNA-Seq提供了主要细胞类型的覆盖，识别了三个不同的脂肪细胞簇，并能够用假时间跟踪脂肪细胞的分化。与Wat相比，脂肪细胞SnRNA-Seq不能匹配脂肪细胞的异质性。SVF scRNA-Seq为非成脂细胞提供了更高的分辨率。这些发现为sc全长转录片段在人类WAT和SVF中的应用提供了关键证据。全长sc/sc RNA-Seq在人类脂肪组织中提供强大的基因覆盖率人类脂肪组织的SnRNA-Seq突出脂肪细胞的异质性SnRNA-Seq在体内跟踪脂肪细胞的分化人SVF的scRNA-Seq提供非脂肪细胞的额外分辨率细胞生物学；Omics；转录组学
White adipose tissue (WAT) is a complex mixture of adipocytes and non-adipogenic cells. Characterizing the cellular composition of WAT is critical for identifying where potential alterations occur that impact metabolism. Most single-cell (sc) RNA-Seq studies focused on the stromal vascular fraction (SVF) which does not contain adipocytes and have used technology that has a 3′ or 5′ bias. Using full-length sc/single-nuclei (sn) RNA-Seq technology, we interrogated the transcriptional composition of WAT using: snRNA-Seq of whole tissue, snRNA-Seq of isolated adipocytes, and scRNA-Seq of SVF. Whole WAT snRNA-Seq provided coverage of major cell types, identified three distinct adipocyte clusters, and was capable of tracking adipocyte differentiation with pseudotime. Compared to WAT, adipocyte snRNA-Seq was unable to match adipocyte heterogeneity. SVF scRNA-Seq provided greater resolution of non-adipogenic cells. These findings provide critical evidence for the utility of sc full-length transcriptomics in WAT and SVF in humans. Full-length sc/sc RNA-Seq provides robust gene coverage in human adipose tissue snRNA-Seq of human adipose tissue highlights adipocyte heterogeneity snRNA-Seq of human adipose tissue tracks adipocyte differentiation in vivo scRNA-Seq of human SVF provides additional resolution of non-adipocyte cells Cell biology; Omics; Transcriptomics