Tracing the structural evolution of eukaryotic ATP binding cassette transporter superfamily.
Tracing the structural evolution of eukaryotic ATP binding cassette transporter superfamily.
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追踪真核 ATP 结合盒转运蛋白超家族的结构进化
DOI：
10.1038/srep16724
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发表时间：
2015-11-18
影响因子：
4.6
通讯作者：
Miao W
中科院分区：
文献类型：
--
作者：
Xiong J;Feng J;Yuan D;Zhou J;Miao W
三磷酸腺苷结合盒(ABC)转运体超家族是膜蛋白中最大的一类。ABC转运蛋白的核心由跨膜区(TMD)和核苷酸结合区(NBD)组成。根据序列相似性和结构域组织，真核生物ABC转运蛋白被分为7个主要家族(ABCA到ABCG)。随着结构域数目和结构的不同，真核生物的ABC转运蛋白呈现出不同的结构：单一结构(NBD或TMD)、ABC2结构(NBD-NBD)、半结构(TMD-NBD或NBD-TMD)和全结构(TMD-NBD-TMD-NBD或NBD-TMD-TMD)。然而，关于不同的ABC转运蛋白基因结构如何进化的研究仍然缺乏。因此，在本研究中，我们全面研究了真核生物ABC转运蛋白的结构进化。7个真核生物ABC转运蛋白家族(A-G)分为A&G组、B组、C&D组和E&F组。在半结构转运蛋白的起始处，至少有4倍于NBD和TMD的融合事件。在FULL和ABC2结构起源中发现了两种融合模式。基于这些发现，我们提出了一个推测的真核生物ABC转运蛋白的结构进化路径，这将增加我们对它们的起源、分化和功能的理解。
The ATP binding cassette (ABC) transporters superfamily is one of the largest classes of membrane proteins. The core of the ABC transporter protein is composed of transmembrane domains (TMDs) and nucleotide binding domains (NBD). Eukaryotes ABC transporters are classified into seven main families (ABCA to ABCG) based on sequence similarity and domain organizations. With different domain number and domain organizations, eukaryote ABC transporters show diverse structures: the single structure (NBD or TMD), the ABC2 structure (NBD-NBD), the half structure (TMD-NBD or NBD-TMD) and the full structure (TMD-NBD-TMD-NBD or NBD-TMD-NBD-TMD). However, studies on how various ABC transporter gene structures evolved is still absent. Therefore, in this study, we comprehensively investigated the structural evolution of eukaryotic ABC transporters. The seven eukaryote ABC transporter families (A to G) fell into three groups: A&G group, B,C&D group and E&F group. There were at least four times the number of NBD and TMD fusion events in the origin of the half structure transporter. Two fusion modes were found in the full and ABC2 structure origination. Based on these findings, we present a putative structural evolutionary path of eukaryote ABC transporters that will increase our understanding on their origin, divergence and function.