Enhancer of decapping proteins 1 and 2 are important for translation during heat stress in Saccharomyces cerevisiae.
Enhancer of decapping proteins 1 and 2 are important for translation during heat stress in Saccharomyces cerevisiae.
复制标题
脱帽蛋白 1 和 2 的增强子对于酿酒酵母热应激期间的翻译非常重要。
DOI：
10.1111/j.1365-2958.2009.06827.x
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发表时间：
2009
影响因子：
3.6
通讯作者：
Thiele,DennisJ
中科院分区：
文献类型：
--
作者：
Neef,DanielW;Thiele,DennisJ
In mammalian andDrosophilacells, heat stress strongly reduces general protein translation while activating cap‐independent translation mechanisms to promote the expression of stress‐response proteins. In contrast, inSaccharomyces cerevisiaegeneral translation is only mildly and transiently reduced by heat stress and cap‐independent translation mechanisms have not been correlated with the heat stress response. Recently we have identified direct target genes of the heat shock transcription factor (HSF), including genes encoding proteins thought to be important for general translation. One gene activated by HSF during heat stress encodes the enhancer of decapping protein, Edc2, previously shown to enhance mRNA decapping under conditions when the decapping machinery is limited. In this report we show that strains lacking Edc2, as well as the paralogous protein Edc1, are compromised for growth under persistent heat stress. This growth deficiency can be rescued by expression of a mutant Edc1 protein deficient in mRNA decapping indicative of a decapping independent function during heat stress. Yeast strains lacking Edc1 and Edc2 are also sensitive to the pharmacological inhibitor of translation paromomycin and exposure to heat stress and paromomycin functions synergistically to reduce yeast viability, suggesting that in the absence of Edc1 and Edc2 translation is compromised under heat stress conditions. Strains lacking Edc1 and Edc2 have significantly reduced rates of protein translation during growth under heat stress conditions, but not under normal growth conditions. We propose that Edc1 and the stress responsive isoform Edc2 play important roles in protein translation during stress.
