Expression and activity of type 1 NAD(P)H dehydrogenase at different growth phases of the cyanobacterium, Synechocystis PCC 6803
Expression and activity of type 1 NAD(P)H dehydrogenase at different growth phases of the cyanobacterium, Synechocystis PCC 6803
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DOI：
10.1111/j.1399-3054.2005.00555.x
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发表时间：
2005-09-01
影响因子：
6.4
通讯作者：
Mi, HL
中科院分区：
文献类型：
--
作者：
Ma, WM;Mi, HL
研究了集胞藻PCC 6803细胞在不同生长期（停滞期、对数期、稳定期和衰退期）中1型NAD（P）H脱氢酶（NDH-1）的表达和活性。使用针对NdhH、NdhI和NdhK的抗体通过蛋白质印迹分析估计的NDH-1的相对量在从滞后期到对数期的生长期间增加超过两倍，然后在对数期之后下降，在15天之后达到最低水平（下降期）。光依赖性NADPH氧化和光系统I（PSI）周围的循环电子流的活动几乎平行的NdhH，NdhI和NdhK在细胞中的量在整个生长阶段的变化。相反，光合放氧和呼吸吸氧的活性在生长的各个阶段没有显著差异，在不同阶段的活性波动在40%以内。这些结果表明，光依赖性NADPH氧化和PSI循环电子流的活性受到NDH-1含量的限制，并且其他因素也限制了光合作用和呼吸速率。
The expression and activity of type 1 NAD(P)H dehydrogenase (NDH-1) were investigated in Synechocystis PCC 6803 cells during different growth phases (i.e. lag, logarithmic, stationary and decline phases). The relative amount of NDH-1, estimated by Western blot analysis using antibodies against NdhH, NdhI and NdhK, increased more than two-fold during growth from the lag to the logarithmic phase and then decreased after the logarithmic phase to reach lowest levels after 15 days (decline phase). The activity of light-dependent NADPH oxidation and cyclic electron flow around photosystem I (PSI) changed nearly in parallel with the amount of NdhH, NdhI and NdhK in cells across the growth phases. In contrast, the activity of photosynthetic O-2 evolution and respiratory O-2 uptake was not significantly different across phases of growth; the fluctuation of the activity at different phases was within 40%. These results suggested that the activity of light-dependent NADPH oxidation and PSI-cyclic electron flow are restricted by the amount of NDH-1 and that other factor(s) are limiting the rates of photosynthesis and respiration.