DIFFERENTIATION OF LENS AND PIGMENT-CELLS IN CULTURES OF NEURAL RETINAL CELLS OF EARLY CHICK-EMBRYOS
DIFFERENTIATION OF LENS AND PIGMENT-CELLS IN CULTURES OF NEURAL RETINAL CELLS OF EARLY CHICK-EMBRYOS
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DOI：
10.1016/0012-1606(77)90124-5
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发表时间：
1977-01-01
影响因子：
2.7
通讯作者：
OKADA, TS
中科院分区：
文献类型：
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作者：
ARAKI, M;OKADA, TS
将3.5日龄鸡胚（20-21期）神经视网膜的分离细胞培养成单层，以检查它们在体外的分化。这些细胞在接种后不久开始活跃生长，并形成汇合片，其中具有长胞质突起的成神经细胞样细胞在8天分化。在16天左右，观察到晶状体样体和色素细胞灶的分化，而神经元结构消失。晶状体小体和色素细胞灶的数量持续增加至30天，当原代培养终止时。δ的增加-晶状体蛋白含量，如通过定量免疫电泳测定使用抗δ-晶状体蛋白的表达与培养物中晶状体小体数量的增加一致。α的量通过与上述相同的技术估计，每个培养物的晶状体蛋白在第16天达到最大值，并在进一步培养期间略有下降。在第2代培养物中也观察到晶状体样体和色素细胞的分化。3.5日龄胚胎视网膜未分化神经上皮细胞在体外至少可分化为神经元细胞、透镜细胞和色素细胞。
Dissociated cells of neural retinas of 3.5 day old chick embryos (stages 20-21) were cultured as a monolayer in order to examine their differentiation in vitro. These cells started to grow actively soon after inoculation and formed a confluent sheet within which neuroblast-like cells with long cytoplasmic processes were differentiated by 8 days. At about 16 days the differentiation of both lentoid bodies and foci of pigment cells was observed, while neuronal structure disappeared. The numbers of lentoid bodies and foci of pigmented cells continued to increase up to 30 days, when primary cultures were terminated. The increase in .delta.-crystallin content, as measured by quantitative immunoelectrophoresis assay using rabbit antiserum against .delta.-crystallin, was consistent with the increase in the number of lentoid bodies in cultures. The amount of .alpha.-crystallin per culture, estimated by the same technique as above, reached a maximum at 16 days and decreased slightly during further culture. The differentiation of both lentoid bodies and pigment cells was observed also in cultures of the 2nd generation. Cells of the undifferentiated neuroepithelium of 3.5 day old embryonic retinas can achieve at least 3 differentiations, neuronal, lens and pigment cells, in vitro.