Immunosuppressive Phenotype of Esophagus Tumors Stroma
Immunosuppressive Phenotype of Esophagus Tumors Stroma
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DOI：
10.1155/2020/5424780
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发表时间：
2020-08-20
影响因子：
3.2
通讯作者：
Gratchev, Alexei
中科院分区：
文献类型：
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作者：
Kovaleva, Olga V.;Rashidova, Madina A.;Gratchev, Alexei
背景肿瘤相关巨噬细胞（TAM）和肿瘤浸润淋巴细胞（TIL）对肿瘤免疫抑制特性的发展有重要作用。在这项研究中，我们进行了食管肿瘤间质免疫细胞的免疫组织化学分析。回顾性收集48例食管鳞状细胞癌（ESCC）患者的石蜡包埋组织标本，进行基质细胞的免疫组化分析。对于巨噬细胞的染色，使用⑶ 68、⑶ 163、⑶ 206、PU. 1和iNOS。对于T细胞检测，使用CD 8、CD 3和FOXP 3。此外，我们对可以在TAM和肿瘤细胞上表达的PD-L1进行了染色。收集临床病理和生存数据，并使用卡方检验和Fisher精确检验、Kaplan-Meier曲线和对数秩检验进行分析。相关分析采用斯皮尔曼等级相关系数。我们发现FOXP 3表达与年龄相关（p=0.042），iNOS表达与疾病分期相关（p=0.044）。此外，FOXP 3和CD 163似乎是预后良好的标志物（HR分别为0.4420，p =0.0325和HR = 0.4447，p =0.0456）。PU.1+和CD 68+巨噬细胞之间存在显著相关性（r=0.833;p
Background. Tumor-associated macrophages (TAMs) and tumor-infiltrating lymphocytes (TILs) contribute significantly to the development of immunosuppressive properties of a tumor. In this study, we performed immunohistochemical analysis of immune cells of esophageal tumors stroma.Methods. Paraffin-embedded tissue specimens from 48 esophageal squamous cell carcinoma (ESCC) patients were retrospectively collected for immunohistochemical analysis of stromal cells. For staining of macrophages, CD68, CD163, CD206, PU.1, and iNOS were used. For T cell detection, CD8, CD3, and FOXP3 were used. Also, we performed staining for PD-L1 that can be expressed on TAMs and tumor cells. Clinicopathological and survival data were collected and analyzed using the chi 2and Fisher exact tests, Kaplan-Meier curves, and the log-rank test. The correlation analysis was performed with Spearman's rank correlation coefficient.Results. We found that FOXP3 expression was associated with age (p=0.042) and iNOS expression was associated with the disease stage (p=0.044). In addition, FOXP3 and CD163 appeared to be markers of good prognosis (HR=0.4420,p=0.0325, andHR=0.4447,p=0.0456, respectively). Significant association between PU.1+ and CD68+ macrophages (r=0.833;p