Massilia rhizosphaerae sp. nov., a rice- associated rhizobacterium with antibacterial activity against Ralstonia solanacearum
Massilia rhizosphaerae sp. nov., a rice- associated rhizobacterium with antibacterial activity against Ralstonia solanacearum
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DOI：
10.1099/ijsem.0.005009
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发表时间：
2021-01-01
影响因子：
2.8
通讯作者：
Wang, Xiangjing
中科院分区：
文献类型：
--
作者：
Li, Chenxu;Cao, Peng;Wang, Xiangjing
从黑龙江省水稻根际土壤中分离到一株对青枯雷尔氏菌（Ralstonia solanacearum）具有抗菌活性的根际细菌，命名为NEAU-GH 312（T）。菌株NEAU-GH 312（T）的细胞为革兰氏染色阴性、需氧、不形成孢子、具有周毛鞭毛和杆状的运动细胞。在28 ℃下孵育2天后，菌落在Reasoner 2A（R2 A）琼脂上呈浅橙子、凸起和半透明。在10-40 ℃、pH 4.0-8.0和0- 5%（w/v）NaCl下在R2 A琼脂上观察到生长。呼吸醌是泛醌Q-8。菌株NEAU-GH 312（T）的主要细胞脂肪酸是C-16：1 omega 7 c和/或C-16：1 omega 6c、C-16：0和C-18：1 omega 7 c和/或C18：1 w 6c。主要极性脂质为磷脂酰甘油、磷脂酰乙醇胺和双磷脂酰甘油。系统发育分析证实菌株NEAU-GH 312（T）在Massilia属中的归属，接近Massilia阿尔维THG-RS 2 OT（98.7%）、Massilia norwichensis NS 9（T）（98.7%）和Massilia kyonggiensis TSA 1（T）（98.6%）的模式菌株。菌株NEAU-GH 312 T具有6.68 Mb的基因组大小和66.3 mol%的平均DNA G+C含量。根据本研究获得的基因型、表型和化学分类学数据，菌株NEAU-GH 312（T）可以被分类为Massilia属的一个新种，并建议将其命名为Massilia roigosphaelum sp. nov.，模式菌株为菌株NEAU-GH 312 T（=DSM 109722 T =CCTCC AB 2019142 T）。
A novel rhizobacterium, designated strain NEAU-GH312(T), with antibacterial activity against Ralstonia solanacearum was isolated from rhizosphere soil of rice (Heilongjiang Province, PR China) and characterized with a polyphasic approach. Cells of strain NEAU-GH312(T) were Gram- stain-negative, aerobic, non- spore-forming, motile with peritrichous flagella and rod-shaped. Colonies were light orange, convex and semi-translucent on Reasoner's 2A (R2A) agar after 2 days of incubation at 28 degrees C. Growth was observed on R2A agar at 10-40 degrees C, pH 4.0-8.0 and with 0-5 % (w/v) NaCl. The respiratory quinone was ubiquinone Q-8. The major cellular fatty acids of strain NEAU-GH312(T) were C-16: 1 omega 7c and/or C-16:1 omega 6c, C-16:0 and C-18:1 omega 7c and/or C18: 1 w6c. The main polar lipids were phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Phylogenetic analyses confirmed the well-supported affiliation of strain NEAU-GH312(T) within the genus Massilia, close to the type strains of Massilia arvi THG-RS2OT (98.7 %), Massilia norwichensis NS9(T) (98.7 %) and Massilia kyonggiensis TSA1(T) (98.6 %). Strain NEAU-GH312T had a genome size of 6.68 Mb and an average DNA G+C content of 66.3 mol%. Based on the genotypic, phenotypic and chemotaxonomic data obtained in this study, strain NEAU-GH312(T) could be classified as representative of a novel species of the genus Massilia, for which the name Massilia rhizosphaerae sp. nov. is proposed, with strain NEAU-GH312T (=DSM 109722T=CCTCC AB 2019142T) as the type strain.