Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons
Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons
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DOI：
10.1007/s00795-022-00330-z
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发表时间：
2022-08-06
影响因子：
1.8
通讯作者：
Imaizumi，Kazuyoshi
中科院分区：
文献类型：
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作者：
Hiramatsu，Noriko;Yamamoto，Naoki;Imaizumi，Kazuyoshi
当再生组织由诱导多能干细胞（iPSC）产生时，有必要跟踪和识别移植的细胞。荧光标记的iPSC合成一种容易追踪的荧光物质。然而，表达的蛋白质不应影响原始基因组序列或多能性。为了解决这个问题，我们创建了一个用于iPSCs基础研究的细胞工具。将来自GFP荧光表达小鼠（GFP-DBA/2小鼠）的虹膜组织衍生的细胞重编程以产生GFP小鼠虹膜衍生的iPSC（M-iris GFP iPSC）。M-iris GFP iPSC表达iPSC的细胞标志物特征，并在分化成三个胚层中显示多能性。此外，当表达GFP时，细胞分化成功能性恢复蛋白和钙结合蛋白阳性细胞。因此，该细胞系将有助于未来对iPSC的研究。
When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs.