Complementary Transcriptomic and Proteomic Analysis Reveals a Complex Network Regulating Pollen Abortion in GMS (msc-1) Pepper (Capsicum annuum L.)
Complementary Transcriptomic and Proteomic Analysis Reveals a Complex Network Regulating Pollen Abortion in GMS (msc-1) Pepper (Capsicum annuum L.)
复制标题
互补转录组学和蛋白质组学分析揭示了 GMS (msc-1) 辣椒 (Capsicum annuum L.) 中调节花粉败育的复杂网络
DOI：
10.3390/ijms20071789
复制
发表时间：
2019-04-11
影响因子：
5.6
通讯作者：
Shen, Huolin
中科院分区：
文献类型：
--
作者：
Cheng, Qing;Li, Ting;Shen, Huolin
辣椒（Capsicum annuum L.）是全球重要的园艺作物。利用基因雄性不育（GMS）株系可以实现高效的商业杂交辣椒种子生产。然而，辣椒GMS的作用机制尚不清楚。在这项研究中，我们使用蛋白质组学和转录组学分析来鉴定与遗传性男性不育相关的蛋白质和基因。在蛋白质组学和转录组学水平上分别鉴定出764个差异表达蛋白（DEPs）和1069个差异表达基因（DEGs），在两个水平上分别检测到52个基因（以下简称“co -DEGs-DEPs”基因）。基因本体（GO）和京都基因与基因组百科全书（KEGG）分析鉴定出13个DEPs和14个DEGs参与绒毡层和花粉发育。在鉴定到的13个DEPs中，8个与花粉外壁形成有关，在可育系16C1369B中均上调表达。在所鉴定的14个基因中，流产的小孢子（AMS）和绒毡层发育和功能缺陷1 （TDF1）参与绒毡层发育，两者都可能受到Msc-1的调控。所有基因均经qRT-PCR检测证实。这些基因的存在提示它们可能在GMS辣椒绒毡层和花粉外壁形成中起作用。在不育系16C1369A中，参与这些过程的大部分关键基因和转录因子均下调。本研究为进一步了解辣椒GMS （msc-1）的分子功能提供了依据。
Pepper (Capsicum annuum L.) is a globally important horticultural crop. Use of the genic male-sterile (GMS) line enables efficient commercial hybrid pepper seed production. However, the mechanisms of pepper GMS functioning remain unclear. In this study, we used proteomic and transcriptomic analysis to identify proteins and genes related to genic male sterility. A total of 764 differentially expressed proteins (DEPs) and 1069 differentially expressed genes (DEGs) were identified in the proteomic and transcriptomic level respectively, and 52 genes (hereafter “cor-DEGs-DEPs” genes) were detected at both levels. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 13 DEPs and 14 DEGs involved in tapetum and pollen development. Among the 13 DEPs identified, eight were involved in pollen exine formation, and they were all up-regulated in the fertile line 16C1369B. For the 14 DEGs identified, ABORTED MICROSPORES (AMS) and DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION1 (TDF1) were involved in tapetum development, and both are possibly regulated by Msc-1. All of these genes were detected and confirmed by qRT-PCR. The presence of these genes suggests their possible role in tapetum and pollen exine formation in GMS pepper. Most key genes and transcription factors involved in these processes were down-regulated in the sterile line 16C1369A. This study provides a better understanding of GMS (msc-1) molecular functioning in pepper.