Click chemistry for fluorescence imaging via combination of a BODIPY-based ‘turn-on’ probe and a norbornene glucosamine
Click chemistry for fluorescence imaging via combination of a BODIPY-based ‘turn-on’ probe and a norbornene glucosamine
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通过结合基于 BODIPY 的“开启”探针和降冰片烯葡萄糖胺进行荧光成像的点击化学
DOI：
10.1039/d2cc05359d
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发表时间：
2022
影响因子：
4.9
通讯作者：
Yan Xu
中科院分区：
文献类型：
--
作者：
Zhiyong He;Takumi Ishizuka;Yoshitaka Hishikawa;Yan Xu
在本研究中，我们合成了一种新的近红外开启BODIPY探针和一种新的藜芦烯修饰的氨基葡萄糖衍生物。该探针显示出显著的NIR荧光发射，具有开启响应，并且可以在荷瘤小鼠中进行肿瘤特异性成像。非天然葡糖胺提供代谢糖工程标记。它可以作为化学标签在细胞上表达，并进一步与荧光染料反应用于细胞标记。这两种衍生物的组合使得能够使用iEDDA反应在体外和体内进行快速和灵敏的细胞成像。
In the present study, we synthesized a novel near-infrared turn-on BODIPY probe and a new norbornene-modified glucosamine derivative. The probe exhibits a significant NIR fluorescence emission with a turn-on response and can perform tumour-specific imaging in tumour-bearing mice. The non-natural glucosamine provides metabolic glycoengineering labelling. It can be expressed on cells as chemical tags and further reacted with fluorescence dyes for cell labelling. The combination of the two derivatives enables quick and sensitive cell imaging in vitro and in vivo using the iEDDA reaction.