Post-training activation of Rac1 in the basolateral amygdala is required for the formation of both short-term and long-term auditory fear memory.
Post-training activation of Rac1 in the basolateral amygdala is required for the formation of both short-term and long-term auditory fear memory.
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训练后激活基底外侧杏仁核中的 Rac1 对于短期和长期听觉恐惧记忆的形成是必需的
DOI：
10.3389/fnmol.2015.00065
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发表时间：
2015
影响因子：
4.8
通讯作者：
Ma L
中科院分区：
文献类型：
--
作者：
Gao Q;Yao W;Wang J;Yang T;Liu C;Tao Y;Chen Y;Liu X;Ma L
Rac1是Rho小GTP酶家族的一员，对成熟神经元突触的形态学变化（包括棘形成和活动依赖性棘扩大）至关重要，而其在相关记忆（如条件性恐惧记忆）形成中的作用尚不清楚。在这里，我们报告说，选择性删除Rac 1兴奋性神经元，但不是在小清蛋白抑制性神经元，受损的短期和长期记忆（STM和LTM）的恐惧条件反射。在关联性恐惧训练之前，在基底外侧杏仁核（BLA）中有条件地敲除Rac 1，这是恐惧记忆获得和储存的关键区域，会损害恐惧记忆。显性负性突变体Rac 1的表达或Rac 1抑制剂NSC23766的注入均阻断了恐惧条件化的STM和LTM。此外，选择性抑制Rac1激活BLA后立即恐惧条件损害STM和LTM，表明恐惧条件诱导的Rac1激活BLA中起着关键作用的STM和LTM的条件性恐惧的形成。
Rac1, a member of the Rho family of small GTPases, is crucial for morphological changes of the mature neuronal synapse including spine formation and activity-dependent spine enlargement, while its role in the formation of associated memories, such as conditioned fear memory, is not clear. Here, we report that selective deletion of Rac1 in excitatory neurons, but not in parvalbumin inhibitory neurons, impaired short- and long-term memories (STM and LTM) of fear conditioning. Conditional knockout of Rac1 before associative fear training in the basolateral amygdala (BLA), a key area for fear memory acquisition and storage, impaired fear memory. The expression of dominant-negative mutant of Rac1, or infusion of Rac1 inhibitor NSC23766 into BLA blocked both STM and LTM of fear conditioning. Furthermore, selective inhibition of Rac1 activation in BLA immediately following fear conditioning impaired STM and LTM, demonstrating that fear conditioning-induced Rac1 activation in BLA plays a critical role in the formation of both STM and LTM of conditioned fear.