miRNA-4317 suppresses human gastric cancer cell proliferation by targeting ZNF322
miRNA-4317 suppresses human gastric cancer cell proliferation by targeting ZNF322
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DOI：
10.1002/cbin.10870
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发表时间：
2018-08-01
影响因子：
3.9
通讯作者：
Huang, Chen
中科院分区：
文献类型：
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作者：
Hu, Xiaoyi;Zhang, Min;Huang, Chen
研究表明miR-4317在肿瘤中表达异常，但miR-4317在肿瘤发生发展中的生物学作用尚不清楚。本研究旨在探讨miR-4317在人胃癌中的作用。采用实时定量聚合酶链式反应技术定量检测miR-4317在临床胃癌标本和细胞系中的表达水平。采用四甲基偶氮唑盐比色法、集落形成实验和细胞周期分析等方法检测miR-4317对胃癌细胞株增殖的影响。结果显示，17例临床胃癌组织中miR-4317的表达明显低于癌旁组织。强制表达miR-4317抑制了胃癌细胞的增殖，阻断了S-G2/M期的转变。生物信息学和双荧光素酶分析证实，ZNF322是miR-4317的直接靶点。沉默ZNF322概括了miR-4317过表达对细胞和分子的影响。这些结果表明，miR-4317至少部分通过靶向和抑制ZNF322抑制胃癌细胞的增殖，可能成为胃癌治疗的靶点。
Studies have shown that miR-4317 is dysregulated in tumor, but the biologic role of miR-4317 in tumor development and progression remains unknown. The present study aimed to investigate the role of miR-4317 in human gastric cancer. Quantitative real-time PCR was used to quantify miR-4317 expression levels in clinical gastric cancer specimens and cell lines. MTT, colony formation and cell cycle assays were performed to identify the contributions of miR-4317 to cell proliferation in gastric cancer cell lines. The results showed that miR-4317 was significantly decreased in 17 clinical gastric cancer specimens compared with adjacent non-tumor stomach tissues. Forced expression of miR-4317 suppressed gastric cancer cell proliferation and blocked S-G2/M transition. Bioinformatics and dual-luciferase reporter assays confirmed that ZNF322 is a direct target of miR-4317. Silencing ZNF322 recapitulated the cellular and molecular effects seen upon miR-4317 overexpression. These findings indicate that miR-4317 represses the proliferation of gastric cancer cell, at least in part, by targeting and suppressing ZNF322 and that it may serve as a therapeutic target for gastric cancer treatment.